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Chulalongkorn Medical Journal

Abstract

Introduction : Several types of brown seaweeds have shown to suppress cancercells by inducing apoptosis and arresting the cell cycle. The brownseaweed from the east coast of the Gulf of Thailand may havepotential effects on cervical cancer as well as ability to induceapoptosis.Objective : To study the anti-proliferative effect and induction of apoptosis byTurbinaria conoides (J. Agardh) K tzing (TCK) on human cervicalcancer cell line.Setting : Burapha UniversityResearch design : An in vitro studyTarget Population : Human cervical cancer cell line (Hela).Method : Fresh samples of Turbinaria conoides (J. Agardh) K tzing (TCK)were extracted and treated HeLa cells. The viable cell number isbased on MTT colorimetric assay. The morphology of apoptoticnuclei were quantified by staining both DAPI and propidium iodide(PI). DNA agarose gel electrophoresis was used for qualitativeanalysis.Result : The extract of TCK inhibited viable HeLa cells in a dose-dependentmanner with IC50 of 20.92 ± 3.15 g/ml associated with roundingand blebbing cells. The apoptotic nuclei in TCK-treated cells were34.92 ± 7.29% (early apoptosis) and 19.56 ± 6.34% (late apoptosis)compared to those in normal cells (45.42 ± 5.97%). In addition,the DNA fragmentation in agarose gel was observed in a dosedependentmanner.Conclusion : The TCK extract induced cell death via morphological and biochemicalevents with particular reference to the induction of apoptosis thatmay have a major impact on the therapy of cancer. Furtherinvestigation is needed to confirm and elucidate detailed mechanismsunderlying the apoptosis.

Publisher

Faculty of Medicine, Chulalongkorn University

First Page

13

Last Page

24

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