Chulalongkorn University Theses and Dissertations (Chula ETD)
Other Title (Parallel Title in Other Language of ETD)
การศึกษาความสัมพันธ์ระหว่างน้ำยาบ้วนปากสมุนไพรกับไมโครไบโอมช่องปาก
Year (A.D.)
2024
Document Type
Thesis
First Advisor
Paswach Wiriyakijja
Faculty/College
Faculty of Dentistry (คณะทันตแพทยศาสตร์)
Degree Name
Master of Science
Degree Level
Master's Degree
Degree Discipline
Oral Biology
DOI
10.58837/CHULA.THE.2024.1411
Abstract
Objective: This study aimed to comparatively evaluate the effects of polyherbal, chlorhexidine (CHX), and normal saline (NCC) mouthwashes on oral microbiome composition, microbial metabolic pathways, and patient-reported outcomes, exploring polyherbal alternatives in response to CHX-associated dysbiosis. Methods: A randomized, double-blind trial was conducted among 27 gingivitis patients, randomly assigned to polyherbal, CHX, or NCC mouthwash groups. Participants used the mouthwash twice daily for 7 days. Saliva samples were analyzed via 16S rRNA sequencing. Microbial diversity (alpha and beta diversity) and composition were statistically evaluated. Differential taxa and pathways were identified using FDR-corrected Wilcoxon tests and log₂ fold-change analysis. Patient-reported outcomes were assessed using numerical rating scales (NRS). Results: The polyherbal mouthwash demonstrated significantly greater patient acceptability compared to CHX and NCC, achieving higher scores in taste, smell, texture, moisture retention, overall satisfaction, and willingness for continued use (p< 0.05). CHX exhibited robust antimicrobial activity but markedly disrupted microbial diversity, significantly increased potentially dysbiotic genera (Streptococcus, Porphyromonas), and altered 23 metabolic pathways associated with microbial dysbiosis. Conversely, the polyherbal mouthwash selectively increased beneficial genera (Amnipila, Absconditabacteriales [SR1], Peptostreptococcus), maintained microbiome diversity, and specifically modulated only two metabolic pathways, notably upregulating L-isoleucine biosynthesis. Conclusion: The polyherbal mouthwash represents a promising alternative to CHX, offering selective microbial modulation, preserved microbiome stability, and significantly improved patient satisfaction. These findings support its integration into routine oral health care as a microbiome-friendly and patient-acceptable solution, warranting further exploration in broader clinical population and extended timeframes.
Other Abstract (Other language abstract of ETD)
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Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Recommended Citation
Wattanawongwan, Waris, "Studying the relationship between polyherbal mouthwash and oral microbiome" (2024). Chulalongkorn University Theses and Dissertations (Chula ETD). 75046.
https://digital.car.chula.ac.th/chulaetd/75046