Elution behavior of carbohydrates using core-shell ion-exchange resin St-60 with different numbers of methylene groups in the porous shell and a constant cross-linking degree of 55%

Authors

Shun-ichi Mitomo
Nao Kodama
Yutaka Inoue

Abstract

It is important to clarify the relationship between the molecular structure of functional groups and the elution of carbohydrates in high-performance liquid chromatography. Herein, ion-exchange resins were synthesized with different numbers of methylene groups (two, four, or six) in the functional unit. The core-shell monomer weight ratio was 40:60 (St-60) and the degree of crosslinking was constant at 55%. The effect of the number of methylene groups on the separation of carbohydrates was examined under strongly alkaline conditions. A mixture of inositol, glucose, fructose, and sucrose was separated using a 0.10 or 0.15 moL/L NaOH eluent at flow rates of 0.3–0.7 mL/min. The retention times for St-60 variants with different numbers of methylene groups (two, four, or six) in the porous layer were found to be nearly the same. The theoretical plate number for glucose and fructose tended to show larger values at flow rates of 0.3 and 0.5 mL/min as the number of methylene groups increased.

DOI

10.56808/3027-7922.2970

Recommended Citation

Mitomo, Shun-ichi; Kodama, Nao; and Inoue, Yutaka (2024) "Elution behavior of carbohydrates using core-shell ion-exchange resin St-60 with different numbers of methylene groups in the porous shell and a constant cross-linking degree of 55%," The Thai Journal of Pharmaceutical Sciences: Vol. 48: Iss. 3, Article 4.
DOI: https://doi.org/10.56808/3027-7922.2970
Available at: https://digital.car.chula.ac.th/tjps/vol48/iss3/4