In vivo anti-acetylcholinesterase, antioxidant activities and acute toxicity of the alkaloid fraction from Hippeastrum reticulatum

Authors

Trang Huyen Xuan Hoang
Quan Van Le
Hoai Thi Nguyen
Huong Thanh Phung

Abstract

Background: Hippeastrum reticulatum (HR) belongs to the Amaryllidaceae, a well-known ornamental family.Objectives: This study was designed to reveal in vivo anti-acetylcholinesterase (AChE) and antioxidant activities and evaluate the acute toxicity of the alkaloid fraction (AF). Methods: The D-galactose-induced aging model and the scopolamine-induced cognitive-deficit model were used for the in vivo tests. The median lethal dose (LD50) of the AF was determined using the Litchfield and Wilcoxon method. Results: On the aging model, the AF at 10 and 15 mg/kg significantly improved the antioxidant markers including superoxide dismutase, glutathione peroxidase, and malonyl dialdehyde. Upon the scopolamine-injected mice, the AF at 10 and 15 mg/kg notably downregulated cerebral AChE in mice. The LD50 of the HR AF is 90.2 mg/kg. Conclusion: These findings provide strong evidence for the safety and advantages of utilizing HR in treating Alzheimer’s disease.

DOI

10.56808/3027-7922.2969

Recommended Citation

Hoang, Trang Huyen Xuan; Le, Quan Van; Nguyen, Hoai Thi; and Phung, Huong Thanh (2024) "In vivo anti-acetylcholinesterase, antioxidant activities and acute toxicity of the alkaloid fraction from Hippeastrum reticulatum," The Thai Journal of Pharmaceutical Sciences: Vol. 48: Iss. 3, Article 3.
DOI: https://doi.org/10.56808/3027-7922.2969
Available at: https://digital.car.chula.ac.th/tjps/vol48/iss3/3