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The Thai Journal of Pharmaceutical Sciences

Abstract

This study will estimate the proximate composition of phytochemicals and study the hypoglycemic effect of Cassia fistula Linn fruit wall extracts. Materials and methods: The in vitro inhibition of alpha-amylase and in vivo streptozotocin induce diabetes model was applied to investigate the anti-diabetic activity. For standardization, Atomic Absorption Spectroscopy and FTIR were used. Results: Proximate analysis values were found to be within the specific limits as recommended by the British Pharmacopeia. The FTIR spectrum and elemental analysis confirmed occurrence of various functional groups and minerals. The phytochemical analysis indicates the higher fraction of the carbohydrates (69.89%) and relatively lesser proportion of the total proteins (10.55%) and the lipids (3.65%). Secondary metabolites such as polysaccharide, flavonoids, polyphenols and glycosaponins were detected in higher proportions in chloroform extract. The percentage inhibition using alpha-amylase assay found to be higher in n-hexane extract that is 93% followed by methanol and chloroform, 81% and 64.7% respectively. In-vivo activity involves the induction of diabetes in rats by streptozotocin and the results showed that a dose of 200mg/kg of n-hexane extract has maximal hypoglycemic effect. Conclusion: The present study supports the anti-diabetic activity of Cassia fistula Linn. and further study on its safety is required.

DOI

10.56808/3027-7922.2791

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