The Thai Journal of Pharmaceutical Sciences
Abstract
Plants have been a primary medicinal source for thousands of years and are utilized time immemorial as popular traditional folk medicine. Rhynchosia cana (R. cana) had therapeutic qualities. In this research, the entire plant R. cana was exposed to maceration with methanol. This plant's methanol extract (MERC) was tested using Gas Chromatography-Mass Spectrometry (GC-MS), which shows 11 main components. Compounds discovered in-silico mechanism of action showed inhibition of α-Amylase and α-glucosidase enzymes. In vitro technique, the MERC also showed α-Amylase and α-glucosidase inhibition. Streptozocin-induced diabetes model evaluated the antihyperglycemic activity in vivo. Single intraperitoneal Streptozotocin injection at a dosage of 150 mg/kg caused experimental diabetes, and animals with fasting blood glucose level (BGL) >200 mg/dL were deemed diabetic. Glibenclamide (5 mg/kg) was a standard medication. Fasting BGL used to evaluate anti-diabetic activity. MERC (200 and 400 mg/kg) demonstrated a substantial decrease in BGL in animal studies. The results obtained in this study suggest that R. cana phytoconstituents can be a safe multi-target therapy to treat diabetes
DOI
10.56808/3027-7922.2787
Recommended Citation
Yempada, Praveena; Marrisetti, Arya Lakshmi; and Battu, Ganga Rao
(2023)
"In-Vivo Antidiabetic Activity and In-Silico Mode of Action of GC-MS Identified Phytoconstituents in Rhynshosia cana,"
The Thai Journal of Pharmaceutical Sciences: Vol. 47:
Iss.
1, Article 12.
DOI: https://doi.org/10.56808/3027-7922.2787
Available at:
https://digital.car.chula.ac.th/tjps/vol47/iss1/12