Anti-inflammatory activity of phenolic-rich fraction of Pandanus tectorius fruits via reduction of NO production on lipopolysaccharide-stimulated RAW264.7 cell lines and formalin-induced paw Oedema

Authors

Yosie Andriani
Nadia Madihah Ramli
Noor Suryani Musa
Kanwal Kanwal
Habsah Mohamad
Hermansyah Amir
Murni Nur Islamiah Kassim
Desy Fitrya Syamsumir
Tengku Sifzizul Tengku Muhammad
Suvik Assaw

Abstract

Background: The current study is the first study that investigated the potential of phenolic-rich fraction of Pandanus tectorius fruits as an anti-inflammatory agent. The fruit is rich in phenolic compounds, especially from the flavonoids group. Phenolics are known to contribute to an anti-inflammatory activity. Objectives: The first objective of the study was to evaluate the anti-inflammatory potency of phenolic-rich fractions of P. tectorius fruits by in vitro and in vivo studies. The second was to investigate the cytotoxicity against RAW264.7 cell lines, antioxidant, and phenolic contents to support the experiment. Materials and Methods: Sample fractionation was done with hexane, ethyl acetate, and methanol successively using column chromatography. Cytotoxicity against RAW264.7 cell lines, phenolic content, and antioxidant property were investigated by MTT, Folin-Ciocalteu, and DPPH assays, respectively. During the in vitro study, the anti-inflammatory potential of P. tectorius fruit fractions was examined by measuring NO production using Griess reagent in LPS-stimulated RAW264.7 cell lines. During the in vivo study, the anti-inflammatory potency of P. tectorius fruit fractions was tested on the Rattus norvegicus paw edema, with inflammation induced using 2.5% formalin. The fraction was given 30 min before the injection of formalin. Results: All fractions, namely, hexane (PHF), ethyl acetate (PEF), and methanol (PMF), had no cytotoxic activity against RAW264.7 cell lines. PEF fraction showed highest phenolic content, antioxidant activity, and highest ability to reduce NO production. Furthermore, paw edema decreased at 6 h by 16.67% in Group C (PEF fraction treatment) and 29.63% in Group D (drug control, ibuprofen) compared to Group B (inflammatory control and formalin treatment). Conclusion: It is confirmed that phenolic-rich fraction of P. tectorius fruits has the potency to reduce inflammation.

DOI

10.56808/3027-7922.2639

First Page

551

Last Page

560

Recommended Citation

Andriani, Yosie; Ramli, Nadia Madihah; Musa, Noor Suryani; Kanwal, Kanwal; Mohamad, Habsah; Amir, Hermansyah; Kassim, Murni Nur Islamiah; Syamsumir, Desy Fitrya; Muhammad, Tengku Sifzizul Tengku; and Assaw, Suvik (2022) "Anti-inflammatory activity of phenolic-rich fraction of Pandanus tectorius fruits via reduction of NO production on lipopolysaccharide-stimulated RAW264.7 cell lines and formalin-induced paw Oedema," The Thai Journal of Pharmaceutical Sciences: Vol. 46: Iss. 5, Article 8.
DOI: https://doi.org/10.56808/3027-7922.2639
Available at: https://digital.car.chula.ac.th/tjps/vol46/iss5/8