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The Thai Journal of Pharmaceutical Sciences

Abstract

The effect of phytochemicals in the aqueous root extracts of Decalepis nervosa Wight & Arn. (ARDN) (Family: Apocynaceae) was investigated on an anticonvulsant action in laboratory animals (mice). The DN roots were extracted using microwave extraction method with aqueous solvent using two parameters, such as temperature variation and extraction time. The phytochemical screening followed by thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC) studies was performed. The pentylenetetrazole and isoniazid models were used with two doses, 250 mg and 500 mg, for the anticonvulsant effects of the ARDN extract. The microwave extraction during 15 min at 60°C temperature revealed the DN root aqueous extract yielded higher (36.20%) than other selected parameters. The phytochemical screening study indicated the existence of alkaloids, flavonoids, glycosides, and phenols, and further TLC, HPTLC studies confirmed the presence of Quercetin and Gallic acid that demonstrated a strong dose dependent anticonvulsant activity. The other biochemical parameters such as lipid peroxidation level showed significant dose-dependent inhibition in malondialdehyde content (29.86%) in mice brain, and also, glutathione content showed significant dose-dependent increased in percentage content (58.22%) in brain of mice. Finally, ARDN’s potential anticonvulsant was first established through the optimized microwave extraction method with the presence of polyphenoilc phytoconstituents.

Publisher

Faculty of Pharmaceutical Sciences, Chulalongkorn University

First Page

307

Last Page

315

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