The Thai Journal of Pharmaceutical Sciences
Abstract
Background: In the present study, a simple, sensitive, rapid, accurate, and precise reverse-phase high-performance liquid chromatography (RP-HPLC) method with diode array detection was developed and validated for simultaneous estimation of Metformin hydrochloride (MET) and Ranolazine (RANO) in a synthetic mixture. Material and Method: The method was developed using isocratic elution mode on a reversed-phase. Chromatographic separation was performed on ACE CN C18 (250 mm, 4.5 mm, 5 μm) column and a mobile phase consisting of methanol: acetonitrile:ammonium formate (45:45:10% v/v/v) with 10% formic acid to adjust the pH to 6.0, at a flow rate of 1.0 ml/min. Detection and quantification of all the analytes were carried out at 230 nm using a photodiode array detector. Result: The method was found to be linear in the range of 1–50 μg/ml for RANO and 2–100 μg/ml for MET. Percentage recovery was found in the range of 99.07–101.53% for RANO and 99.59–100.45% for MET. The RSD of precision and repeatability was found lower than 1% for each drug. Validation of the method was carried out as per International Council on Harmonization guideline Q2 (R1). Conclusion: The proposed RP-HPLC method can be highly suitable for the analysis of RANO and MET without interference.
Publisher
Faculty of Pharmaceutical Sciences, Chulalongkorn University
First Page
287
Last Page
292
Recommended Citation
Patel, Ashok B.; Gol, Dhruvanshi A.; Makwana, Rahul L.; Vyas, Amitkumar J.; Patel, Ajay I.; Patel, Nilesh K.; Faldu, Shital D.; and Lumbhani, Arvind N.
(2022)
"Analytical method for simultaneous estimation of ranolazine and metformin hydrochloride by validated RP-HPLC-DAD method,"
The Thai Journal of Pharmaceutical Sciences: Vol. 46:
Iss.
3, Article 6.
Available at:
https://digital.car.chula.ac.th/tjps/vol46/iss3/6