The Thai Journal of Pharmaceutical Sciences
Abstract
Objective: The present study investigated the antibacterial activities of asam gelugur (Garcinia atroviridis) fruit extracts against two Gram-negative bacterial strains (Escherichia coli (gi: CP033762.1) and Salmonella enterica (gi: KX355299.1)). Materials and Methods: The powder form of G. atroviridis fresh fruit was extracted with 95% ethanol (2:4 L) (w/v). The mixtures were filtered and evaporated using a rotary evaporator at 50–55°C. High-performance liquid chromatography (HPLC) was used to measure hydroxycitric acid (HCA) in the extract. The antibacterial assay was determined by disk diffusion method, the minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC). Results: HPLC analysis measured approximately 495.01 ± 2.13 mg/g (49.50%) of HCA in the crude extract. The antibacterial analysis revealed that the highest concentration (200,000 μg/ml) of ethanol extracts inhibited the growth of both bacteria, with the diameters of inhibition zones of E. coli and S. enterica being 33.11 and 31.58 mm, respectively. The MIC value effective against both E. coli and S. enterica was 12,500 μg/ml. The MBC value which completely killed both bacteria was 25,000 μg/ml. Conclusion: It can be concluded that the G. atroviridis extract shows antibacterial property against E. coli and S. enterica.
DOI
10.56808/3027-7922.2580
First Page
346
Last Page
352
Recommended Citation
Niyomdecha, Atchara; Wattanachant, Chaiyawan; Rattanawut, Jessada; Plodpai, Patimaporn; and Ngampongsai, Wanwisa
(2022)
"Antibacterial activity of ethanol extract of asam gelugur (Garcinia atroviridis) fruits from Southern Thailand,"
The Thai Journal of Pharmaceutical Sciences: Vol. 46:
Iss.
3, Article 14.
DOI: https://doi.org/10.56808/3027-7922.2580
Available at:
https://digital.car.chula.ac.th/tjps/vol46/iss3/14