The Thai Journal of Pharmaceutical Sciences
Synthesis and cytotoxic activity of N-(2,4-dichloro)benzoyl-N’-phenylthiourea against human breast cancer cell line
Introduction: Urea- and thiourea-based compound has shown a potency to be further developed as anticancer compound. Objective: Another novel phenylthiourea analog (N-(2,4-dichloro)benzoyl-N’-phenylthiourea) was synthesized through Schotten-Baumann reaction followed by the assessment of its cytotoxic activity. Methods: N-(2,4-dichloro)benzoyl-N’-phenylthiourea was synthesized using N-Phenylthiourea and 2,4-dichlorobenzoyl chloride as starting materials. The reaction started at low temperature for 30 min followed by reflux for 8 h to yield target compound. Cytotoxicity assay was then performed against MCF-7, T47D, and Vero normal cell line. Results: The compound has been synthesized and its structure has been verified using infrared, 1H-nuclear magnetic resonance (NMR), 13C-NMR, various 2D NMR, and mass spectra. Furthermore, it is shown that the synthesized compound possesses better cytotoxicity profile than hydroxyurea. Conclusion: N-(2,4-dichloro)benzoyl-N’-phenylthiourea is a potential thiourea analog as anticancer agent, albeit further research is needed.
Faculty of Pharmaceutical Sciences, Chulalongkorn University
Kesuma, Dini; Medika, Anwar; and Yuniarta, Tegar Achsendo
"Synthesis and cytotoxic activity of N-(2,4-dichloro)benzoyl-N’-phenylthiourea against human breast cancer cell line,"
The Thai Journal of Pharmaceutical Sciences: Vol. 46:
2, Article 7.
Available at: https://digital.car.chula.ac.th/tjps/vol46/iss2/7