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The Thai Journal of Pharmaceutical Sciences

Abstract

Objectives: This study compared the hepatoprotective activity of Pterocarpus santalinoides cold maceration extract (PSCME) with P. santalinoides Soxhlet extract (PSSE). Materials and Methods: Phytochemical constituents of both extracts were determined. 45 albino rats, randomly assigned into 9 groups (1–9) of 5 rats each, were used for the study. Liver damage was induced in groups 1–8 using carbon tetrachloride (CCl4). Group 1 served as untreated control, Groups 2–7 were treated with 50, 250 or 500 mg/kg PSCME or PSSE, Group 8 was treated with 100 mg/kg silymarin, while Group 9 served as normal control. Treatment was done orally for 15 days. Serum enzyme activities, proteins, cholesterol and bilirubin levels, relative liver weight and liver histomorphology were evaluated. Results: There were higher concentrations of phenols and saponins in PSCME than in PSSE. 250 mg/kg dose of both PSCME and PSSE significantly (P < 0.05) protected hepatocellular integrity, enhanced hepatic bilirubin clearance, ameliorated hepatomegaly, and also led to less severe vacoulation and necrosis of hepatocytes in the rats given CCl4. Conclusion: Extracts of P. santalinoides obtained by cold maceration and Soxhlet extraction techniques are of nearly equal hepatoprotective potency. The heat applied during Soxhlet extraction had no adverse effect.

Publisher

Faculty of Pharmaceutical Sciences, Chulalongkorn University

First Page

442

Last Page

450

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