The Thai Journal of Pharmaceutical Sciences
Abstract
The properties of Croton persimilis Müll.Arg. and Antidesma puncticulatum Miq.— interesting plant in the family Euphorbiaceae were investigated. The ethanol crude plant extracts were established for phytochemicals screening, antioxidants activities, anticancer in five important human cancer cell lines (MCF-7, MDA-MB-231, HeLa, HepG2, and NCI-H187), and cytotoxic activity in normal cell line (hTERT-HME1). The leaves extracts of C. persimilis Müll. Arg. contained flavonoids, saponins, terpenoids, and tannins. The total phenolic content properties were found in 43.40 ± 1.36 mg Gallic Acid Equivalents (GAE)/g crude extract. They also showed lowest antioxidant activity through 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay (IC50>1,000 μg/ml) and 2.4.2 ferric reducing antioxidant power (FRAP) assay (369.84 ± 2.39 μM/g of crude extract). Whereas, the raw fruits of A. puncticulatum Miq. contained saponins and tannins. The total phenolic content properties were found in 193.47 ± 4.63 mg GAE/g crude extract. The antioxidant activity showed better IC50 via DPPH assay (IC50 = 51.50 ± 3.30 μg/ml) and FRAP assay (964.67 ± 5.88 μM/g of crude extract). The leaves extracts of C. persimilis Müll Arg had the greatest inhibition activity against MCF-7 and CaCO2, while the raw fruits extract of A. puncticulatum Miq. had low growth inhibition against in all cancerous cells. Thus, both extracts are potentially sources of natural antioxidants. C. persimilis Müll Arg may be a potential compound in anticancer.
Publisher
Faculty of Pharmaceutical Sciences, Chulalongkorn University
First Page
394
Last Page
399
Recommended Citation
Rattanapunya, Siwalee; Sumsakul, Wiriyaporn; Bunsongthae, Akharasit; and Jaitia, Samart
(2021)
"In vitro antioxidants and anticancer
activity of crude extract isolates from
Euphorbiaceae in Northern Thailand,"
The Thai Journal of Pharmaceutical Sciences: Vol. 45:
Iss.
5, Article 11.
Available at:
https://digital.car.chula.ac.th/tjps/vol45/iss5/11