The evaluation on anti-platelet and antithrombosis activities of Cinnamomum sintoc bark extract

Authors

Nanang Fakhrudin
Tri Wiyono
Arbie Ristanto Putra
Sitarina Widyarini
Arief Nurrochmad

Abstract

Introduction: One of the factors contributing to cardiovascular diseases is platelet aggregation. Although several antiplatelet agents have been introduced into clinical practices, discovery of antiplatelet agents is still challenging. Plants from the genus of Cinnamomum were previously reported to have antiplatelet activity. This study aimed to determine the activity of Cinnamomum sintoc (CS) bark extract inhibiting platelet aggregation and thrombosis. Materials and Methods: Antiplatelet activity of CS bark methanolic extract (CS methanolic extract [CSME]) (5–300 μg/mL) was determined by measuring the ability of the extract to inhibit platelet aggregation induced by epinephrine. Meanwhile, the antithrombosis activity was determined by calculating the survival mice and by measuring the thrombus formation in the mice lung capillaries after challenging with thrombus-inducing agent, combination of collagen, and epinephrine. Results: The results showed that CSME inhibited platelet aggregation induced by epinephrine with the IC50 of 117.9 ± 8.8 μg/mL. However, CSME at low, medium, and high doses (100, 400, and 600 mg/kg BW, respectively) failed to protect the mice from death and paralysis after thrombotic challenge. Conclusions: CSME demonstrated an in vitro antiplatelet activity, but it failed to protect thrombosis in the thrombotic mice.

DOI

10.56808/3027-7922.3055

Recommended Citation

Fakhrudin, Nanang; Wiyono, Tri; Putra, Arbie Ristanto; Widyarini, Sitarina; and Nurrochmad, Arief (2025) "The evaluation on anti-platelet and antithrombosis activities of Cinnamomum sintoc bark extract," The Thai Journal of Pharmaceutical Sciences: Vol. 43: Iss. 4, Article 5.
DOI: https://doi.org/10.56808/3027-7922.3055
Available at: https://digital.car.chula.ac.th/tjps/vol43/iss4/5