The Thai Journal of Pharmaceutical Sciences
Abstract
Objective: The purpose of the study is to evaluate the hepatoprotective effects of Phikud Navakot (PN) extract against hydrogen peroxide (H2O2)-induced oxidative stress in HepG2 cells. Materials and Methods: The cells were treated with concentrations of PN up to 1 mg/ml before incubation with H2O2. Cell viability and reactive oxygen species (ROS) generation were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and 2′,7′-dichlorofluorescein diacetate assays, respectively. Oxidative defense mechanisms were investigated by measuring glutathione (GSH) levels and activities of antioxidant enzymes. Expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) proteins was investigated by Western blot analyses. Results: The results showed that PN extract at concentrations of 0.001–0.1 mg/ml significantly improved cell viability and prevented ROS generation induced by H2O2 in HepG2 cells. The extract also increased the activities of antioxidant enzymes (catalase, glutathione peroxidase, glutathione reductase, and superoxide dismutase), total GSH, GSH, and the GSH/glutathione disulfide (GSSG) ratio, but decreased GSSG. Pre-treatment with PN prevented the decreases in Nrf2 and HO-1 protein levels caused by H2O2-induced oxidative stress. Conclusion: This study highlights the hepatoprotective effect of PN against H2O2-induced oxidative stress and the associated mechanisms.
DOI
10.56808/3027-7922.3052
Recommended Citation
Chiangsom, Abhiruj; Maniratanachote, Rawiwan; Meksuriyen, Duangduen; Luechapudiporn, Rataya; Kulthong, Kornphimol; Aueviriyavit, Sasitorn; Oda, Shingo; Yokoi, Tsuyoshi; and Lawanprasert, Somsong
(2025)
"Protective effect of Phikud Navakot extract against hydrogen peroxide-induced oxidative stress in HepG2 cells,"
The Thai Journal of Pharmaceutical Sciences: Vol. 43:
Iss.
4, Article 2.
DOI: https://doi.org/10.56808/3027-7922.3052
Available at:
https://digital.car.chula.ac.th/tjps/vol43/iss4/2
