Eriodictyol attenuates hydrogen peroxide-induced cell death in neuronal SH-SY5Y cells

Authors

Saowarose Thongin
Narudol Teerapattarakan
Hattaya Benya-aphikul
Varisa Pongrakhananon
Boonchoo Sritularak
Ratchanee Rodsiri

Abstract

Background: Eriodictyol, a flavonoid isolated from Dendrobium ellipsophyllum, provides health benefits in several oxidative stress-related diseases. Objective: This study aimed to determine the protective effect and mechanisms of eriodictyol on hydrogen peroxide (H2O2)-induced cell death in neuronal SH-SY5Y cells. Methods: The protective effect of eriodictyol against H2O2 was evaluated by resazurin assay. Reactive oxygen species (ROS) and the mitochondrial membrane potential were detected by 2’-7’ dichlorodihydrofluorescein diacetate and tetramethylrhodamine ethyl ester assays, respectively. Finally, the effects of eriodictyol on antioxidant enzyme expression and the mitochondria-mediated apoptotic cascade were determined using Western blot analysis. Results: Pre-treatment with eriodictyol suppressed cell death mediated by H2O2. Intracellular ROS and the loss of mitochondrial membrane potential induced by H2O2 were significantly reduced following eriodictyol treatment. Western blot analysis revealed that H2O2 enhanced the expression of BAX/Bcl-2, cytochrome c, cleaved caspase-3, and caspase-3, which was, in turn, attenuated in the presence of eriodictyol. Furthermore, pre-treatment with eriodictyol extensively promoted the expression of antioxidant enzymes including superoxide dismutase and catalase, indicating the significant neuroprotective effect of eriodictyol through an antioxidantdependent mechanism. Conclusion: Eriodictyol shows a protective effect against H2O2 -induced neuronal cell death in SH-SY5Y cells through a decrease of ROS production, an increase in antioxidant enzyme expression, the protection of mitochondrial function, and the inhibition of the mitochondria-mediated apoptotic cascade. Our data provide scientific information for the development of potent therapeutic agents for neurodegenerative diseases.

DOI

10.56808/3027-7922.3044

Recommended Citation

Thongin, Saowarose; Teerapattarakan, Narudol; Benya-aphikul, Hattaya; Pongrakhananon, Varisa; Sritularak, Boonchoo; and Rodsiri, Ratchanee (2025) "Eriodictyol attenuates hydrogen peroxide-induced cell death in neuronal SH-SY5Y cells," The Thai Journal of Pharmaceutical Sciences: Vol. 43: Iss. 3, Article 4.
DOI: https://doi.org/10.56808/3027-7922.3044
Available at: https://digital.car.chula.ac.th/tjps/vol43/iss3/4