Antibacterial activitry of crude extracts from edible mushrooms Pleurotus citrinopileatus and Tricholoma crassum Berk

Authors

Prontep Chomcheon
Bancha Kheawkum
Pornsinee Sriwiset
Suphak Dulsamphan
Chalernkieat Dulsamphan

Abstract

The purpose of this research was to study antibacterial activity of crude extracts derived from the culture broths of two edible mushrooms, Pleurotus citrinopileatus and Tricholoma crassum Berk, cultivated in submerged fermentation. Potato dextrose broth (PDB) and yeast extract sucrose broth (YES) were used as culture media. Each culture filtrate was extracted and evaporated to yield a crude extract. Antibacterial activities against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923 were determined by paper disc diffusion assay. Crude extract (1 mg) of P. citrinopileatus culturing PDB showed the widest inhibition zones against E. coli ATCC 25922 (34.47 ± 0.50 mm) and S. aureus ATCC 25923 (33.73 ± 0.23 mm). Minimum inhibitory concentrations of this active crude extract against E. coli ATCC 25922 and S. aureus ATCC 25923, as determined by the broth microdilution method, were 5.0 mg/ml and 2.5 mg/ml, respectively. Crude extract of T. crassum culturing PDB showed weak activity against S. aureus ATCC 25923. YES broth was found to be inapplicable for cultivation of both mushrooms to produce antibacterial metabolite.

DOI

10.56808/3027-7922.3324

First Page

107

Last Page

111

Recommended Citation

Chomcheon, Prontep; Kheawkum, Bancha; Sriwiset, Pornsinee; Dulsamphan, Suphak; and Dulsamphan, Chalernkieat (2013) "Antibacterial activitry of crude extracts from edible mushrooms Pleurotus citrinopileatus and Tricholoma crassum Berk," The Thai Journal of Pharmaceutical Sciences: Vol. 37: Iss. 2, Article 4.
DOI: https://doi.org/10.56808/3027-7922.3324
Available at: https://digital.car.chula.ac.th/tjps/vol37/iss2/4