Lactobacillus plantarum T23/3 as a potential probiotic strain isolated from faces

Authors

Titpawan Nakpheng
Rosainee Kha
Neelam Balekar
Sanae Kaewnopparat
Teerapol Srichana

Abstract

The present study was undertaken to evaluate the properties of Lactobacillus plantarum T23/3 isolated from the feces of healthy elderly volunteer. The L. plantarum T23/3 showed better tolerance property to bile acid, simulated gastric fluid (SGF) and simulated intestinal fluid (SIF), and exhibited antimicrobial activity against tested enteropathogens. It formed macroscopic granules with surface hydrophobicity and co-aggregated with tested microbial strain. It was resistant to some antibiotics used for treatment of diarrhea so there is possibility to co-administer L. plantarum as probiotic with antidiarrheal therapy. Furthermore, adhesion and significant potential for decreasing adhesion of pathogens (E. coli 2601 and V. cholera 3194) to Caco-2 cells were observed in these experiments. The stability profile of L. plantarum T23/3 reflected viability (upto 12 months) in enteric coated capsules containing lactose. While the freeze-dried lactobacilli loaded into the EudragitL-100 coated capsule did not release the probiotic content in SGF pH 1.2 over 2 h but dissolved, released the L. plantarum 23/3 instantly in the SIF at pH 7.4. Our study provides evidence that L. plantarum T23/3 has a promising future to protect humans from gastrointestinal infections and affirms to serve as a probiotic.

DOI

10.56808/3027-7922.3317

First Page

83

Last Page

93

Recommended Citation

Nakpheng, Titpawan; Kha, Rosainee; Balekar, Neelam; Kaewnopparat, Sanae; and Srichana, Teerapol (2012) "Lactobacillus plantarum T23/3 as a potential probiotic strain isolated from faces," The Thai Journal of Pharmaceutical Sciences: Vol. 36: Iss. 3, Article 1.
DOI: https://doi.org/10.56808/3027-7922.3317
Available at: https://digital.car.chula.ac.th/tjps/vol36/iss3/1