•  
  •  
 

Abstract

Background: Increased concerns over the potential health impacts on consumers from widespread pyrethroid use in agriculture have necessitated the implementation of maximum residue limits (MRLs) on food products. In Thailand, while such regulations exist, their enforcement remains incomplete.  The study aims to monitor pyrethroid contamination in Khon Kaen market vegetables and evaluate the risks posed to human health.

Method: A total of 100 samples representing ten different fresh vegetable types were collected from May to July 2024. Pyrethroid residues in vegetables collected from both structured and unstructured markets in Khon Kaen, Thailand, were analyzed using gas chromatography (GC) with a micro electron capture detector (μECD). Health risks for both short-term and long-term exposure were calculated by combining the detected pyrethroid residue levels with estimated dietary intake data, utilizing a relevant database.

Results: Samples of Chinese kale contained the highest levels of lambda-cyhalothrin detected at 0.71 mg/kg and bifenthrin at 0.70 mg/kg. The structured markets within Muang District had a high prevalence of detectable residues from pyrethroid groups. An evaluation of the short-term (sHI) and long-term (cHQ) health risks associated with the consumption of these vegetables indicates potential health risks exceeding acceptable thresholds, particularly among children.

Conclusion: This investigation reveals pyrethroid contamination in vegetables from the Khon Kaen province markets in Thailand. It is vital to reduce contamination to ensure food security as well as educate consumers about this contamination to increase their food safety.

Keywords: Pyrethroids, Pesticides, Maximum Residue Limits, Vegetables, Insecticide residues, Health risk assessment

References

[1] Agriculture Department. Thailand. Summary of import of hazardous substances 2024. Bangkok: Department of Agriculture; 2024 [cited 2025 Nov]. Available from: https://www.doa.go.th/th/.

[2] Laohaudomchok W, Nankongnab N, Siriruttanapruk S, Klaimala P, Lianchamroon W, Ousap P, et al. Hum Ecol Risk Assess 2021;27(5):1147—69. https://doi.org/10.1080/10807039.2020.1808777.

[3] Baudry J, Rebouillat P, Samieri C, Berlivet J, Kesse-Guyot E. Dietary pesticide exposure and non-communicable diseases and mortality: a systematic review of prospective studies among adults. Environ Health 2023 Oct 31;22(1):76. https://doi.org/10.1186/s12940-023-01020-8.

[4] Rebouillat P, Vidal R, Cravedi JP, Taupier-Letage B, Debrauwer L, Gamet-Payrastre L, et al. Prospective association between dietary pesticide exposure profiles and type 2 diabetes risk in the NutriNet-Santé cohort. Environ Health 2022 May 25;21(1):57. https://doi.org/10.1186/s12940-022-00862-y.

[5] Rebouillat P, Vidal R, Cravedi JP, Taupier-Letage B, Debrauwer L, Gamet-Payrastre L, et al. Prospective association between dietary pesticide exposure profiles and postmenopausal breast-cancer risk in the NutriNet-Santé cohort. Int J Epidemiol 2021;50(4):1184—98. https://doi.org/10.1093/ije/dyab015.

[6] Goyal R, Spence KA, Borodinsky LN. From Neural Tube Formation Through the Differentiation of Spinal Cord Neurons: Ion Channels in Action During Neural Development. Front Mol Neurosci 2020;13:1—11. https://doi.org/10.3389/fnmol.2020.00062.

[7] Shen H, Bocksteins E, Kondrychyn I, Snyders D, Korzh V. Functional antagonism of voltage-gated K+ channel αsubunits in the developing brain ventricular system. Development 2016 Nov 15;143(22):4249—60. https://doi.org/10.1242/dev.140467.

[8] Elser BA, Hing B, Stevens HE. A narrative review of converging evidence addressing developmental toxicity of pyrethroid insecticides. Crit Rev Toxicol 2022 May;52(5): 371—88. https://doi.org/10.1080/10408444.2022.2122769.

[9] Prasopsuk J, Srisawangwong W, Seelarak P, Khattiyaphutthimet N. Health Risk Assessment of Pesticide Residues in Vegetables Collected from Upper Northeast Thailand. Thai Agricultural Research Journal 2025;43(1): 78—88. https://doi.org/10.14456/thaidoa-agres.2025.7 [in Thai].

[10] Harnpicharnchai K, Chaiear N, Charerntanyarak L. Residues of organophosphate pesticides used in vegetable cultivation in ambient air, surface water and soil in Bueng Niam Subdistrict, Khon Kaen, Thailand. Southeast Asian J Trop Med Publ Health 2013;44(6):1088—97.

[11] Sapbamrer R, Sittitoon N, Thongtip S, Chaipin E, Sutalangka C, Chaiut W, et al. Acute health symptoms related to perception and practice of pesticides use among farmers from all regions of Thailand. Front Public Health 2024 Jan 8;11:1296082. https://doi.org/10.3389/fpubh.2023.1296082.

[12] Ahamad A, Kumar J. Pyrethroid pesticides: An overview on classification, toxicological assessment and monitoring. J Hazard Mater Adv 2023;10:100284. https://doi.org/10.1016/j.hazadv.2023.100284.

[13] Matsuo N. Discovery and development of pyrethroid insecticides. Proc Jpn Acad Ser B Phys Biol Sci 2019;95(7): 378—400. https://doi.org/10.2183/pjab.95.027.

[14] Tang W, Wang D, Wang J, Wu Z, Li L, Huang M, et al. Pyrethroid pesticide residues in the global environment: An overview. Chemosphere 2018 Jan;191:990—1007. https://doi.org/10.1016/j.chemosphere.2017.10.115.

[15] Osaili TM, Al-Natour MQ, Al-Abboodi AR, Alkarasneh AY, Darra NE, Khazaal S, et al. Detection and risk associated with organochlorine, organophosphorus, pyrethroid and carbamate pesticide residues in chicken muscle and organ JOURNAL OF HEALTH RESEARCH 2026;40(1):335—345 343 ORIGINAL STUDY meats in Jordan. Food Control 2023;144:1—12. https://doi.org/10.1016/j.foodcont.2022.109355.

[16] Chen C, Qian Y, Liu X, Tao C, Liang Y, Li Y. Risk assessment of chlorpyrifos on rice and cabbage in China. Regul Toxicol Pharmacol 2012 Feb;62(1):125—30. https://doi.org/10.1016/j.yrtph.2011.12.011.

[17] Food Agriculture Oraganzation [FAO]. Maximum Residue Limits (MRLs) 2026 [cited 2025 Nov] .Available from: https:// www.fao.org/fao-who-codexalimentarius/codex-texts/maximum-residue-limits/en/.

[18] Biostatistics WDW. A foundation of analysis in the health science. New York: John Wiley & Sons; 1995.

[19] Wanwimolruk S, Phopin K, Boonpangrak S, Prachayasittikul V. Food safety in Thailand 4: comparison of pesticide residues found in three commonly consumed vegetables purchased from local markets and supermarkets in Thailand. PeerJ 2016 Sep 1;4:e2432. https://doi.org/10.7717/peerj.2432.

[20] Leuprasert L, Taneepanichskul S, Monmora S, Puangtapa S, Jifan S, Siriwong W, et al. Pesticide Use and Pesticide Related Symptom Prevalence in Chinese Kale Farmers in Northeast Thailand. J Health Res 2014;28(4):255—62.

[21] Nasuk C, Jannarong P, Sukreeta S. Determination and health risk assessment of pesticide residues through fresh vegetables and fruits consumption in northeast of Thailand. SciTech Research Journal 2023;6(3):1—19 ([in Thai]).

[22] Poochada W, Uengchuen K, Junggoth R, Donprajum T, Seesophon S, Sanpool O, et al. Current High Prevalence of Intestinal Parasitic Contamination in Fresh Vegetables in Northeast Thailand. Am J Trop Med Hyg 2024 Nov 26;112(2): 314—8. https://doi.org/10.4269/ajtmh.24-0234.

[23] Steinwandter H. Universal 5-min on-line method for extracting and isolating pesticide residues and industrial chemicals. Z für Anal Chem 1985;322(8):752—4. https://doi.org/10.1007/BF00489393.

[24] European Commission. Pesticide Residues. [cited 2025 Nov]. Available from: https://ec.europa.eu/food/plant/pesticides/eu-pesticides-database/start/screen/mrls.

[25] National Bureau of Agricultural Commodity and Food Standards, Thailand. Pesticide Residues: Maximum Residue Limits. Bangkok: National Bureau of Agricultural Commodity and Food Standards 2016:4—53 [cited 2025 Nov]. Available from: https://certify.dld.go.th/images/pdfs/law/annonec-moac/inma02.pdf.

[26] Food Innovation & Regulation Network [FIRN]. Thai agricultural standard. Bangkok : FIRN; 2023 [in Thai]. Avaliable from: https://firn.or.th/%E0%B8%A1%E0%B8%B2%E0%B8%95%E0%B8%A3%E0%B8%90%E0%B8%B2%E0%B8%99%E0%B8%AA%E0%B8%B4%E0%B8%99%E0%B8%84%E0%B9%89%E0%B8%B2%E0%B9%80%E0%B8%81%E0%B8%A9%E0%B8%95%E0%B8%A3/.

[27] Prapamontol T, Hongsibsong S, Naksen W, Kerdnoi T, Kawichai S, Polyiem W, et al. Multiple Pesticide Residues Found in Vegetables and Fruits from Rural and Urban Markets in Upper Northern Thailand. CMUJ Net Sci 2020; 20(1):1—25. https://doi.org/10.12982/CMUJNS.2021.002.

[28] World Health Oraganization [WHO]. Pesticide residues in food: joint FAO/WHO meeting on pesticide residues. WHO pesticide residues series. Rome, Italy: WHO; 2021 [cited 2025 Nov]. Available from: http://apps.who.int/pesticideresidues-jmpr-database/Home/Range/All.

[29] Meftaul IM, Venkateswarlu K, Annamalai P, Parven A, Megharaj M. Glyphosate use in urban landscape soils: Fate, distribution, and potential human and environmental health risks. J Environ Manag 2021 Aug 15;292:112786. https://doi.org/10.1016/j.jenvman.2021.112786.

[30] Minstry of Public Health, Thailand, Department of Health. Dietary reference intake For thais 2020. Nontaburi. Minstry of Public Health, Thailand, Department of Health; 2020. Available from: https://nutrition2.anamai.moph.go.th/webupload/6x22caac0452648c8dd1f534819ba2f16c/202101/m_news/9457/176096/file_download/a72848fb8557732574f6afd877a0a0a3.pdf [in Thai].

[31] Phulkerd S, Thapsuwan S, Thongcharoenchupong N, Gray R, Chamratrithirong A. Sociodemographic differences affecting insufficient fruit and vegetable intake: a population-based household survey of Thai people. J Health Res 2020;34(5):419—29. https://doi.org/10.1108/JHR-07-2019-0150.

[32] Zhang Q, Ying Z, Tang T, Guo B, Gu S, Fu L, et al. Residual characteristics and potential integrated risk assessment of synthetic pyrethroids in leafy vegetables from Zhejiang in China — Based on a 3-year investigation. Food Chem 2021 Dec 15;365:130389. https://doi.org/10.1016/j.foodchem.2021.130389.

[33] Wanwimolruk S, Kanchanamayoon O, Phopin K, Prachayasittikul V. Food safety in Thailand 2: Pesticide residues found in Chinese kale (Brassica oleracea), a commonly consumed vegetable in Asian countries. Sci Total Environ 2015 Nov 1;532:447—55. https://doi.org/10.1016/j.scitotenv.2015.04.114.

[34] Suntudrob J, Jongmevasna W, Payanan T, Srikote R, Wittayanan W. Monitoring of pesticide residues in domestic vegetables in Thailand during 2015. Asia-Pacific Journal of Science and Technology 2018;23(4):1—8.

[35] Pakvilai N, Prapamontol T, Thavornyutikarn P, Mangklabruks A, Chantara S, Santasup C. Residues of synthetic pyrethroid pesticides in vegetables, fruit, sediment and water from an intensive agricultural area (Fang district, Chiang Mai, Thailand), vol. 167. WIT Transactions on Ecology and The Environment; 2011. p. 201—10. https://www.witpress.com/elibrary/wit-transactions-on-ecologyand-the-environment/167/23028.

[36] Prasopsuk J, Suwit L, Promkhambut A, Iwai CB. Food safety risk assessment of pesticide residues in Chinese kale grown in Khon Kaen province, northeast Thailand. Agric Nat Resour 2020;54(4):343—50. https://doi.org/10.34044/j.anres.2020.54.4.01.

[37] European Food Safety Authority [EFSA]. Pesticide residues in food: latest figures released 2024. [cited 2025 Nov]. Available from: https://www.efsa.europa.eu/en/news/pesticide-residues-food-latest-figures-released.

[38] Pesticise Action Network Europe [PAN Europe]. The return of EU-banned pesticides: dangerous residues found in imported food. Brussels: PAN Europe; 2025 [cited Nov 2025]. Available from: https://www.pan-europe.info/node/3975.

[39] Prasopsuk J, Laohasiriwong S, Promkhambut A, Iwai CB. Food safety risk assessment of pesticide residues in Chinese kale grown in Khon Kaen province, northeast Thailand. Agric Nat Resour 2020;54:343—50. https://doi.org/10.34044/j.anres.2020.54.4.01.

[40] Amekawa Y. Can a public GAP approach ensure safety and fairness? A comparative study of Q-GAP in Thailand. J Peasant Stud 2013;40(1):189—217. https://doi.org/10.1080/03066150.2012.746958.

[41] El-Sheikh EA, Ramadan MM, El-Sobki AE, Shalaby AA, McCoy MR, Hamed IA, et al. Pesticide Residues in Vegetables and Fruits from Farmer Markets and Associated Dietary Risks. Molecules 2022 Nov 21;27(22):8072. https://doi.org/10.3390/molecules27228072.

[42] Nisha US, Khan MS, Prodhan MD, Meftaul IM, Begum N, Parven A, et al. Quantification of Pesticide Residues in Fresh Vegetables Available in Local Markets for Human Consumption and the Associated Health Risks. Agronomy 2021;11(9):1804. https://doi.org/10.3390/agronomy11091804.

[43] Kunno J, Ong-Artborirak P, Taneepanichskul N, Siriwong MGRW. Effect of pyrethroid insecticides exposure in relation to pyrethroid metabolite and GABA concentration of young children, Bangkok Thailand. Hum Ecol Risk Assess 2019;27(1):1—14. https://doi.org/10.1080/10807039.2019.1689098.

[44] Breckenridge CB, Holden L, Sturgess N, Weiner M, Sheets L, Sargent D, et al. Evidence for a separate mechanism of toxicity for the Type I and the Type II pyrethroid insecticides. Neurotoxicology 2009 Nov;30(Suppl 1):S17—31. https://doi.org/10.1016/j.neuro.2009.09.002.344 JOURNAL OF HEALTH RESEARCH 2026;40(1):335—345 ORIGINAL STUDY

[45] Liu J, Schelar E. Pesticide exposure and child neurodevelopment: summary and implications. Workplace Health Saf 2012 May;60(5):235—42. https://doi.org/10.1177/216507991206000507. quiz 243.

[46] Senneca O, Scherillo F, Nunziata A. Thermal degradation of pesticides under oxidative conditions. J Anal Appl Pyrolysis 2007;80(1):61—76. https://doi.org/10.1016/j.jaap.2007.01.002.

[47] Phopin K, Norkaew SWC, Isarankura-Na-Ayudhya JBC. Boiling, Blanching, and Stir-Frying Markedly Reduce Pesticide Residues in Vegetables. Foods 2022 May 18;11(10): 1463. https://doi.org/10.3390/foods11101463.

Share

COinS