•  
  •  
 

Abstract

Background: Global interest in herbal bioactive compounds as alternative therapies has increased in recent years; however, scientific data on their distribution across species and on the active constituents of local medicinal plants remain limited. This study examined medicinal herbs cultivated by farmers in Mae Chai District, Phayao Province, Thailand, and assessed their antioxidant activities, as well as their phenolic and vitamin C contents, to support the development of evidence-based alternative medicine.

Methods: This study employed a mixed-methods design combining scientific experimentation with qualitative approaches. A total of 34 key informants participated. Data were collected through surveys, interviews, reflecting discussions, and Global Positioning System (GPS)-based field mapping conducted from May to December 2023. The concentrations of phenolic compounds and vitamin C, as well as antioxidant activities (FRAP, ABTS, and DPPH), were determined in herbal products.

Results: Thunbergia laurifolia (TL), Vernonia cinerea (VC), and Zingiber officinale (ZO) were processed into herbal teas sourced from farmer plantations identified by geographic coordinates. Among the tested species, Curcuma longa (357 mg/g) and Cissus quadrangularis (255 mg/g) showed the highest FRAP activity, while Cassia alata (95, 27 µg/mL) and T. laurifolia (150, 55 µg/mL) exhibited the strongest DPPH and ABTS antioxidant capacities. TL and CA had the highest phenolic and vitamin C concentrations, respectively.

Conclusion: These results confirm that key local herbs used for capsules and herbal teas possess antioxidant activity, supporting their role in sustainable health systems and evidence-based Thai traditional medicine.

Keywords: Herbs, Anti-oxidant, Thai Traditional Medicine, GIS mapping, Phytochemistry, Thailand

References

[1] World Health Organization [WHO]. Traditional medicine strategy: 2014-2023. [cited 2023 March 3]. Available from: https://www.who.int/publications/i/item/97892.41506096.

[2] Tipsurat P. Study of local herbal plants and folk wisdom for wage: case study Kaeang Krung National Park, Suratthani Province. Songkla: Prince of Songkla University; 2016.

[3] Thailand, Department of Thai traditional and alternative medicine. National master plan for the development of Thai herbs, No. 1, 2017—2021. [Cited 2021 May 1]. Available from: https://www.old.dtam.moph.go.th/index.php/th/download/1672-dl0021.html.

[4] Tongboonkuea Y. Diversity and utilization of medicinal plants in Tham Phet-Tham Thong Forest Park Takhlee JOURNAL OF HEALTH RESEARCH 2026;XX:1—11 9 ORIGINAL STUDY District, Nakhon Sawan Province. [Cited 2022 June 5]. Available from: https://frc.forest.ku.ac.th/frcdatabase/bulletin/Document/C00647.pdf.

[5] Mueangchang W. Factors related to self treatment with herbal remedies of people in Mea Chai District, Phayao Province. Bangkok: Thammasart University; 2016.

[6] Chang KT. Introduction to geographic information systems. 9th ed. New York: McGraw-Hill; 2019.

[7] Qayum DA, Lynn A, Arya R. Traditional knowledge system based GIS mapping of antimalarial plants: spatial distribution analysis. J Geogr Inf Syst 2014;6:478—91. https://doi.org/0.4236/jgis.2014.65041.

[8] Mennis J, Yoo EE. Geographic information science and the analysis of place and health. Trans GIS 2018;22(3):842—54. https://doi.org/10.1111/tgis.12337.

[9] Geo-Informatics and Space Technology Development Agency [GISTDA]. Space technology and geo-informatics. Bangkok: GISTDA; 2009. p. 1—8.

[10] Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, et al. Oxidative stress: harms and benefits for human health. Oxid Med Cell Longev 2017;2017:8416763. https://doi.org/10.1155/2017/8416763.

[11] Widowati W, Wargasetia TL, Zakaria TM, Gunadi MS, Halim N, Santiadi S, et al. Antioxidant activities of RoJa Herbal Tea: Combination of Rosella Flower (Hibiscus sabdariffa L.) and Ginger Rhizome (Zingiber officinale Rosc.). Pharm Sci Res 2023;10(3):172—9. https://doi.org/10.7454/psr.v10i3.1305.

[12] Lonan P, Ariyabukalakorn V, Yoysungnoen B, Singsai K, Praphasawat R, Sangkham S, et al. Hepatoprotective effects of Gac (Momordica cochinchinensis) aril extract in acetaminophen-induced liver injury: modulation of oxidative stress, inflammation, and glucose metabolism. J Exp Pharmacol 2025;17:527—43. https://doi.org/10.2147/jep.S517411.

[13] Surarit W, Jansom C, Lerdvuthisopon N, Kongkham S, Hansakul P. Evaluation of antioxidant activities and phenolic subtype contents of ethanolic bran extracts of Thai pigmented rice varieties through chemical and cellular assays. Int J Food Sci Technol 2015;50(4):990—8. https://doi.org/10.1111/ijfs.12703.

[14] Adebiyi OE, Olayemi FO, Ning-Hua T, Guang-Zhi Z. In vitro antioxidant activity, total phenolic and flavonoid contents of ethanol extract of stem and leaf of Grewia carpinifolia. Beni Suef Univ J Basic Appl Sci 2017;6(1):10—4. https://doi.org/10.1016/j.bjbas.2016.12.003.

[15] Tantipaiboonwong P, Pintha K, Chaiwangyen W, Chewonarin T, Pangjit K, Chumphukam O, et al. Antihyperglycaemic and anti-hyperlipidaemic effects of black and red rice in streptozotocin-induced diabetic rats. Sci Asia 2017;43(5):281—8. https://doi.org/10.2306/scienceasia1513-1874.2017.43.281.

[16] Satpathy L, Pradhan N, Dash D, Baral PP, Parida SP. Quantitative determination of vitamin C concentration of common edible food sources by redox titration using iodine solution. Lett Appl NanoBioSci 2021;10(3):2361—9. https://doi.org/10.33263/LIANBS103.23612369.

[17] Dwivedi MK, Shyam BS, Shukla R, Sharma NK, Singh PK. GIS mapping of antimalarial plants based on traditional knowledge in Pushparajgarh Division, District Anuppur, Madhya Pradesh, India. J Herbs, Spices, Med Plants 2020; 26(4):356—78. https://doi.org/10.1080/10496475.2020.1747583.

[18] Mashayekhan A, Pourmajidian M, Jalilvand H, Gholami M, Teimouri MS. Economic importance and GIS mapping of medicinal plants in Iran: a case study of Darkesh. J Appl Sci Environ Manag 2016;20:646—50. https://doi.org/10.4314/jasem.v20i3.19.

[19] Rice-Evans C, Miller N, Paganga G. Antioxidant properties of phenolic compounds. Trends Plant Sci 1997;2(4):152—9. https://doi.org/10.1016/S1360-1385(97)01018-2.

[20] Sudha P, Zinjarde SS, Bhargava SY, Kumar AR. Potent αamylase inhibitory activity of Indian ayurvedic medicinal plants. BMC Compl Alternative Med 2011;11:5. https://doi.org/10.1186/1472-6882-11-5.

[21] Lindsay DG, Astley SB. European research on the functional effects of dietary antioxidants - EUROFEDA. Mol Aspect Med 2002;23(1—3):1—38. https://doi.org/10.1016/s0098-2997(02)00005-5.

[22] Tan BL, Norhaizan ME, Liew WP, Sulaiman Rahman H. Antioxidant and oxidative stress: a mutual interplay in agerelated diseases. Front Pharmacol 2018;9:1162. https://doi.org/10.3389/fphar.2018.01162.

[23] Pamulaparthi A, Banala M, Swamy Nanna R. Plant regeneration via somatic embryogenesis in an ethnomedicinal plant senna alata (L.) Roxb. Biotechnol J Int 2016;15(4):1—8. https://doi.org/10.9734/BBJ/2016/28504.

[24] Fatmawati S, Yuliana Purnomo AS, Abu Bakar MF. Chemical constituents, usage and pharmacological activity of Cassia alata. Heliyon 2020;6(7):e04396. https://doi.org/10.1016/j.heliyon.2020.e04396.

[25] Moeurng S, Posridee K, Kamkaew A, Thaiudom S, Oonsivilai A, Oonsivilai R. Identification of pheophytin a and hydroxy pheophytin a from Rang Chuet (Thunbergia laurifolia Linn.) as potent NQO-1 inducers in liver cells. Foods 2024;13(10). https://doi.org/10.3390/foods13101443.

[26] Stankovic M, Terzic J, Stefanovic O. Synergistic antibacterial activity of Curcuma longa L. and Urtica dioica L. extracts and preservatives. Kragujev J Sci 2019;41:107—16. https://doi.org/10.5937/KgJSci1941107S.

[27] Tanvir EM, Hossen MS, Hossain MF, Afroz R, Gan SH, Khalil MI, et al. Antioxidant properties of popular turmeric (Curcuma longa) varieties from Bangladesh. J Food Qual 2017;2017(1):8471785. https://doi.org/10.1155/2017/8471785.

[28] Oboh G, Akinyemi AJ, Ademiluyi AO. Antioxidant and inhibitory effect of red ginger (Zingiber officinale var. Rubra) and white ginger (Zingiber officinale Roscoe) on Fe(2+) induced lipid peroxidation in rat brain in vitro. Exp Toxicol Pathol 2012;64(1—2):31—6. https://doi.org/10.1016/j.etp.2010.06.002.

[29] Chatterjee S, Chatterjee S, Dey K, Dutta S. Study of Antioxidant activity and immune stimulating potency of the ethnomedicinal plant, Cassia alata (L.) Roxb. Med Aromat Plants (Los Angel) 2013;2(4):1000131. https://doi.org/10.4172/2167-0412.1000131.

[30] Abubakker N. Evaluation of enzymic and non-enzymic antioxidant levels in vernonia cinerea extracts. Asian J Pharm Clin Res 2018;11(7):167—70. https://doi.org/10.22159/ajpcr.2018.v11i7.22109.

[31] Yeh HY, Chuang CH, Chen HC, Wan CJ, Chen TL, Lin LY. Bioactive components analysis of two various gingers (Zingiber officinale Roscoe) and antioxidant effect of ginger extracts. LWT - Food Sci Technol (Lebensmittel-Wissenschaft -Technol) 2014;55(1):329—34. https://doi.org/10.1016/j.lwt.2013.08.003.

[32] Thakur R, Yadav K, Khadka KB. Study of Antioxidant, antibacterial and anti-inflammatory activity of cinnamon (Cinamomum Tamala), ginger (Zingiber Officinale) and turmeric (Curcuma Longa). Am J Life Sci 2013;1(6):273—7. https://doi.org/10.11648/j.ajls.20130106.16.

[33] Hongsing P, Palanuvej C, Ruangrungsi N. In vitro biological activities of thunbergia laurifolia stem and leaf with reference to rosmarinic acid. Songklanakarin J Sci Technol 2020; 42(3):615—20.

[34] Thinh NH, Wichittrakarn P, Laosinwattana C, Teerarak M. Total polyphenol content and antioxidant activity of the extracts from Thunbergia laurifolia Lindl. leaves. Int J Agric Technol 2017;13:1325—32.

[35] Jantarach N, Mueangchang W, Prathumtet J, Munkong N. Beneficial effects of red rice bran extract on brown adipose tissue whitening, inflammation, and oxidative stress in mice with obesity induced by a 10 JOURNAL OF HEALTH RESEARCH 2026;XX:1—11 ORIGINAL STUDY high-fat diet. Pharm Sci Asia 2023;50(3):257—65. https://doi.org/10.29090/psa.2023.03.23.105.

[36] Munkong N, Ruxsanawet K, Ariyabukalakorn V, Mueangchang W, Sangkham S, Silangirn P, et al. Hepatoprotective effects of Elaeagnus latifolia fruit extract against acetaminophen-induced hepatotoxicity in mice: mechanistic insights. J Funct Foods 2024;114:106077. https://doi.org/10.1016/j.jff.2024.106077.

[37] Mahdavi H, Hadadi Z, Ahmadi M. A review of the antioxidation, anti-inflammatory and anti-tumor properties of Curcumin. Trad Integr Med 2017;2(4):188—95.

[38] Ajayi BE, Odueke YA, Ibrahim FM, Ighodaro M, Bamgbose JT. Contraceptive efficacy of aqueous extract of Xylopia aethiopica (Dunal) A. Rich. fruit in female sprague dawley rats. Trad Integr Med 2023;8(4):362—9. https://doi.org/10.18502/tim.v8i4.14484.

Share

COinS