Ya-Hok-Sing: bioactive substance components, in vitro toxicity, and a case series

Authors

Khareeyoh Asae, Traditional Thai Medical Research and Innovation Center, Faculty of Traditional Thai Medicine, Prince of Songkla University, Songkhla 90110, Thailand
Oratai Neamsuvan, Traditional Thai Medical Research and Innovation Center, Faculty of Traditional Thai Medicine, Prince of Songkla University, Songkhla 90110, Thailand
Nantiya Joycharat, Traditional Thai Medical Research and Innovation Center, Faculty of Traditional Thai Medicine, Prince of Songkla University, Songkhla 90110, ThailandFollow

Abstract

Background: Ya-Hok-Sing is a traditional Thai polyherbal medicine used by Singhanakhon healers, Thailand. This study aimed to provide scientific evidence supporting the traditional use of Ya-Hok-Sing as an anti-fever remedy.

Methods: The chemical fingerprinting and in vitro toxicity of Ya-Hok-Sing were investigated using the Liquid Chromatography-Quadrupole time-of-flight-Mass spectrometry (LC-QTOF-MS) method and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The clinical efficacy of Ya-Hok-Sing for fever relief was examined using a case series of five patients following treatment from a traditional Thai healer.

Results: The mass-spectrometry-total ion chromatogram (MS‑TIC) for Ya-Hok-Sing revealed the presence of thirty-six compounds, including twenty-one phenolics and fifteen organic acids. Ya-Hok-Sing was non-toxic at the highest tested concentration of 80 µg/mL in vero cells and mouse fibroblast cells. The results of the case series indicated that three patients out of five tested positive for COVID-19. The two remaining patients were diagnosed with influenza by a traditional healer. For the first two days, each patient took 90 mL of Ya-Hok-Sing every three hours. Starting on day 3, each patient took Ya-Hok-Sing three times daily (90 mL each) before meals for one week. All patients improved clinically after three to five days and recovered from fever and co-morbidities by six to nine days. Quality of life assessment showed that the patients had better mean quality of life scores after treatment (106.40 ± 7.90), compared to before treatment (62.00 ± 5.34).

Conclusion: Our findings supported the folk medicinal properties of Ya-Hok-Sing for the treatment of fever.

Keywords: Ya-Hok-Sing, Chemical fingerprinting, Toxicity, Thailand

Recommended Citation

Asae K, Neamsuvan O, Joycharat N. Ya-Hok-Sing: bioactive substance components, in vitro toxicity, and a case series. J Health Res. 2025; 39(1):-. DOI: https://doi.org/10.56808/2586-940X.1120

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