The mathematical model predicts peripheral blood CD34+ cell yield and blood volume processing for peripheral blood stem cell apheresis.

Authors

• Rattaporn Vichitratchaneekorn, King Chulalongkorn Memorial HospitalFollow
• Kanokpol Siriphanthong, King Chulalongkorn Memorial HospitalFollow
• Kanokwan Chinbordee, National Blood Centre, Thai Red Cross SocietyFollow
• Phandee Watanaboonyongcharoen, Chulalongkorn UniversityFollow
• Ponlapat Ronjnuckarin, Chulalongkorn UniversityFollow

Abstract

Introduction: The accurate prediction of stem cell yields is important for planning peripheral blood stem cell (PBSC) collection. This study aimed to develop a formula forecasting required blood volume processing to ensure adequate CD34+ cell products.

Methods: Data from donors and patients who underwent PBSC apheresis conducted at a tertiary care hospital were reviewed. BD FACSCanto™ II and Navios EX flow cytometry machines were used to determine the pre-collection CD34+ cell counts (preCD34).

Results: Of 50 donors and 85 patients, 145 leukapheresis procedures were performed. Linear regression models accurately predicted absolute CD34+ cell yields from pre-apheresis values, supporting optimal procedural planning. Subgroup analysis identified gender, but not body weight, as a significant factor influencing collection efficiency, with lower yields in female individuals. To ensure adequate stem cell collection, gender-specific formulas were derived to calculate the required blood volume processing: For males: Volume process (L) = [(Dose CD34 (10⁶Cells/kg) * Body Weight Recipient (kg)) + 21]/ 0.44 * PreCD34(Cells/μL); For females: Volume process (L) = [(Dose CD34 (10⁶Cells/kg) * Body Weight Recipient (kg)) – 48]/ 0.28 * PreCD34(Cells/μL). Furthermore, a strong correlation was observed between the two enumeration platforms (r = 0.981), with Bland-Altman analysis confirming minimal bias and narrow variability. No significant difference in preCD34 was detected.

Conclusion: This applying PreCD34 count and gender-specific predictive modeling offers valuable tools to enhance procedural efficiency, generating appropriate processed volume for adequate stem cell collection. Furthermore, the importance of standardizing CD34+ enumeration methods was underscored.

DOI

10.56808/2673-060X.5783

First Page

1

Last Page

22

Recommended Citation

Vichitratchaneekorn, Rattaporn; Siriphanthong, Kanokpol; Chinbordee, Kanokwan; Watanaboonyongcharoen, Phandee; and Ronjnuckarin, Ponlapat () "The mathematical model predicts peripheral blood CD34+ cell yield and blood volume processing for peripheral blood stem cell apheresis.," Chulalongkorn Medical Journal: Vol. 70: Iss. 5, Article 4.
DOI: https://doi.org/10.56808/2673-060X.5783
Available at: https://digital.car.chula.ac.th/clmjournal/vol70/iss5/4