Abstract
ABSTRACT:
Background and objective: The present study aimed to evaluate the antioxidant and hypolipidemic effects of ethanol extract of Nardostachys Jatamansi (N.J) in a high-fat diet-induced obesity model in male Wistar albino rats.Materials and methods:36 male Wistar albino rats were randomly divided into six groups, each comprising six animals: Group I received a normal diet for 9 weeks, while Group II was fed a high-fat diet (HFD) for the same period. Groups III–VI were also given HFD throughout. From week 5, Group III received rosuvastatin (10 mg/kg), and Groups IV–VI were treated with Nardostachys Jatamansi. extract at 100, 200, and 300 mg/kg, respectively, for 4 weeks. All treatments were administered orally. At the end of the study, animals were fasted overnight, anesthetized, and blood was collected for lipid and haematological analysis. Organs were excised for histological evaluation.Statistical analysis: Data were expressed as mean ± SD and analysed using one-way ANOVA followed by Tukey’s post hoc test. SPSS v19 was used, with P < 0.05 considered significant.Results: Nardostachys Jatamansi (100, 200, 300 mg/kg) dose-dependently reversed HFD-induced increases in body weight, fat pad size, organ weight, total cholesterol, triglycerides, LDL, and VLDL, while restoring HDL levels. Elevated WBC, lymphocyte counts, and serum urea, creatinine, and uric acid were also normalized. Histological improvements in the liver suggest antioxidant and bioactive effects of Nardostachys Jatamansi.Conclusion: Our results showed that herbal drug Nardostachys Jatamansi effectively ameliorates high-fat diet-induced dyslipidemia through its potent antioxidant activity, which helped reduce oxidative stress and restore lipid homeostasis.
DOI
10.56808/2673-060X.5795
First Page
Ameliorative effect of ethanolic extract of Nardostachys Jatamansi against high-fat diet-induced dyslipidemia in rats INTRODUCTION: Obesity is now recognized as one of the most widespread metabolic disorders [1] globally, with dyslipidemia being a major related complication. While obesity has a multifactorial origin, high-fat diet (HFD) consumption remains a key driving factor. Even without visible obesity, excessive dietary fat promotes lipid accumulation in both adipose and non-adipose tissues, triggering cellular damage and death through lipotoxicity.[2] Cardiovascular diseases are the primary causes of morbidity and mortality linked to dyslipidemia.[3] In light of the increasing global reliance on high-fat diets, there is a pressing need to explore natural compounds or dietary supplements that can mitigate or prevent their harmful metabolic effects. Nardostachys jatamansi, commonly known as Jatamansi, is a well-known natural herb widely recognized in orthodox systems like Ayurveda, valued for its diverse therapeutic effects.[4] Studies have highlighted its pharmacological activities, including lipid-lowering [5], hematopoietic [6], antipyretic [7], cardioprotective [8], and hepatoprotective [9] properties. These benefits are largely due to its rich phytochemical content, such as lignans, sesquiterpenoids, neolignans, and terpenoids [10], which possess strong antioxidant potential against oxidative stress and dyslipidemia. This study aims to evaluate the efficacy of the ethanolic extract of Nardostachys Jatamansi against HFD-induced dyslipidemic model. MATERIALS AND METHODS: Animals Male adult Wistar rats (6 weeks old, weighing 250 ± 20 g) were obtained from an authorized breeder. Before the experiment, the animals were
Last Page
26. J. F. Baker, E. Krishnan, L. Chen,and H.R.Schumacher,“Serum uric acid and cardiovascular disease: recent developments, and wheredotheyleaveus?”TheAmericanJournal of Medicine,vol. 118, no. 8, pp. 816–826, 2005. 27. Tsushima Y, Nishizawa H, Tochino Y, Nakatsuji H, Sekimoto R, Nagao H, et al. Uric acid secretion from adipose tissue and its increase in obesity. J Biol Chem. 2013 Sep 20;288(38):27138-27149. doi: 10.1074/jbc.M113.485094. Epub 2013 Aug 2. PMID: 23913681; PMCID: PMC3779712. 28. Unruh D, Srinivasan R, Benson T, Haigh S, Coyle D, Batra N, et al. Red Blood Cell Dysfunction Induced by High-Fat Diet: Potential Implications for Obesity-Related Atherosclerosis. Circulation. 2015 Nov 17;132(20):1898-908. doi: 10.1161/CIRCULATIONAHA.115.017313. Epub 2015 Oct 14. PMID: 26467254; PMCID: PMC4772773. 29. Wang YM, Zhang B, Xue Y, Li ZJ, Wang JF, Xue CH, et al. The mechanism of dietary cholesterol effects on lipids metabolism in rats. Lipids Health Dis. 2010 Jan 14;9:4. doi: 10.1186/1476-511X-9-4. PMID: 20070910; PMCID: PMC2820024. 30. Singh M, Khan MA, Y T K, Ahmad J, Fahmy UA, Kotta S, et al. Effect of Nardostachys jatamansi DC. on Apoptosis, Inflammation and Oxidative Stress Induced by Doxorubicin in Wistar Rats. Plants (Basel). 2020 Nov 15;9(11):1579. doi: 10.3390/plants9111579. PMID: 33203171; PMCID: PMC7734586.
Recommended Citation
PREETHI, UMANATH; Sumathy, Govindaraj; and Shanmugam, Nathiya
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"Ameliorative effect of ethanolic extract of Nardostachys Jatamansi against high fat diet induced dyslipidemia in rats,"
Chulalongkorn Medical Journal: Vol. 70:
Iss.
5, Article 3.
DOI: https://doi.org/10.56808/2673-060X.5795
Available at:
https://digital.car.chula.ac.th/clmjournal/vol70/iss5/3