Morphological and Biological Properties of Platelet Rich Fibrin Membrane Producing from Different Centrifugation Protocols

Authors

Vannarut Satitpitakul MD, Chulalongkorn UniversityFollow
Sarisa Tangkhunchaisaeng, chulalongkorn universityFollow

Abstract

Background: Platelet-rich fibrin (PRF) membranes represent a promising tool for corneal epithelial disease treatment, though a standardized protocol remains unestablished. Objective: This study aimed to evaluate the morphological and biological properties of Platelet-Rich Fibrin (PRF) membranes producing from different centrifugation protocols Methods: Four distinct low speed centrifugation protocols (100g/5min, 200g/5min, 100g/10min, and 200g/10min) were established for PRF production from 3 healthy volunteers. We assessed fibrin and membrane dimensions, white blood cell and platelet concentrations, kinetic release of growth factors (TGF-, PDGF-BB, VEGF, IGF-1), and inflammatory cytokines (IL-6, IL-1, TNF-). In vitro degradation and bacterial contamination were also examined. Results: All four centrifugation protocols consistently produced PRF membranes with high platelet concentration. Distinct release patterns were also observed for each growth factor and cytokine. The 100g/5min protocol produced the highest platelet concentration. The PRF fibrin matrix effectively encapsulated growth factors, enabling their sustained release over a minimum of 10 days. No bacterial contamination was detected in any protocol. Conclusion: Our findings definitively demonstrate that centrifugation protocol significantly impacts the biological properties, particularly platelets and growth factor concentration, of PRF membranes. The 100g force, 5-minute protocol yielded the highest platelet concentration, offering significant therapeutic potential for ocular surface diseases compared to other protocols.

DOI

10.56808/2673-060X.5681

First Page

Original Articles Title: Morphological and Biological Properties of Platelet Rich Fibrin Membrane Producing from Different Centrifugation Protocols Running title: Autologous platelet rich fibrin membrane protocols Author names: Sarisa Tangkhunchaisaeng1, Krit Pongpirul2, Vannarut Satitpitakul3, 4 Author affiliations: 1 Department of Ophthalmology, Faculty of Medicine, Chulalongkorn University and King Chulalongkorn Memorial Hospital, Bangkok, Thailand 2 Department of Preventive and Social Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand 3 Center of Excellence for Cornea and Stem Cell Transplantation, Department of Ophthalmology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand 4 Excellence Center for Cornea and Stem Cell Transplantation, Department of Ophthalmology, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand Corresponding Author: Vannarut Satitpitakul, MD 1873 Rama4 Road Pathumwan Bangkok 10330 THAILAND Vannarut.S@chula.ac.th Funding: Ratchadapisek Somphot Research Fund (Funding number GA067/057)

Last Page

BB, VEGF, IL-1, IL-6 and TNF-α from all centrifugation protocols in all observation times (all P>0.05).

Recommended Citation

Satitpitakul, Vannarut MD and Tangkhunchaisaeng, Sarisa (2025) "Morphological and Biological Properties of Platelet Rich Fibrin Membrane Producing from Different Centrifugation Protocols," Chulalongkorn Medical Journal: Vol. 69: Iss. 5, Article 7.
DOI: https://doi.org/10.56808/2673-060X.5681
Available at: https://digital.car.chula.ac.th/clmjournal/vol69/iss5/7