Abstract
Background: C. sappan heartwood extract is a good antioxidant potential natural compound against oxidative stress. A few data have been reported with those of value using cell base assay, especially in normal human lung fibroblast cells.
Objective: The heartwood extracts of C. sappan were obtained with different ethanol concentrations and investigated on the antioxidant capacity on human lung fibroblast MRC-5.
Method: The crude extracts were subjected to antioxidant activity assays based on free radical scavenging activity by Chemi-based assay and cell-based assay (in-vitro lipid peroxidation, CAT and SOD) using MRC-5 cells.
Result: The study shown that the total phenolic content and antioxidant effect of C. sappan heartwood extracts were significantly dependent on the ethanol concentration used for extraction. The crude extract prepared by 75% ethanol is the best of antioxidant properties. This extract contains amounts of total phenols (741.8 mg GAE/g C. sappan extract), with IC50 values (2.4 µg/mL in DPPH assay) and reduced activities enzyme and inhibited lipid peroxidation on MRC-5 cells (13.35, 11.83, and 10.71 U/mg protein in CAT assay, 2.21, 2.13, and 1.99 U/mg protein in SOD assay, and 0.97 and 1.13 nM MDA/mg protein in lipid peroxidation assay).
Conclusion: This result support significant a good antioxidant potential of sappan heartwood extracts against oxidative stress and could serve as dietary supplements providing healthy life.
DOI
10.56808/2673-060X.5472
Recommended Citation
kongkham, supranee and Kaenu, Aylada
(2025)
"Antioxidant activity of Caesalpinia sappan heartwood extracts against hydrogen peroxide-induced oxidative damage in MRC-5 cell lines,"
Chulalongkorn Medical Journal: Vol. 69:
Iss.
3, Article 6.
DOI: https://doi.org/10.56808/2673-060X.5472
Available at:
https://digital.car.chula.ac.th/clmjournal/vol69/iss3/6