Abstract
Background: Grapes are the most widely consumed fruit around the world. Grape seed comprises a group of polyphenols are known to have a broad pharmacological and therapeutic activities. The grape seed extract was known to have counteract against oxidative stress by its free radical scavenging activity.
Objectives: To identify the major phytocompounds of grape seed methanol extract and to evaluate its antioxidant activity
Methods: In this study, the methanol extract of the grape seed was subjected to Gas Chromatography-Mass Spectrometry to identify the major phytochemical compounds and further antioxidant activity was also evaluated by DPPH method using AOAC (Association of Official Analytical Chemist) guidelines.
Results: Totally 10 phytochemical compounds (1,3,5-Benzenetriol, 1,3-Benzenediol, Benzoic acid, Reticuline, 2-Hydroxy-5-methylisophthalaldehyde, 4-Vinylbenzene-1,2-diol, Cyclohexane carboxylic acid, Eicosanoic acid, Octadecanoic acid and 2,6,10,14,18-Pentamethyl-2,6,10,14,18-eicosapentaene) were identified from the grape seed extract. The antioxidant activity of the grape seed extract was found to be 212.6 µg/kg by DPPH method.
Conclusion: Phytochemical profiling of grape seed extract revealed the presence of phenols (1,3-Benzenediol, 4-Vinylbenzene-1,2-diol), polyphenol (1,3,5-Benzenetriol), phenolic acid (Benzoic acid), alkaloid (Reticuline), aldehyde (2-Hydroxy-5-methylisophthalaldehyde), straight-chain saturated fatty acid (Eicosanoic acid, Octadecanoic acid), and carboxylic acid (Cyclohexane carboxylic acid). Of these phytocompounds, Benzoic acid and Reticuline are potent natural antioxidants with an antioxidant capacity of 212.6 µg/kg.
DOI
10.56808/2673-060X.5459
First Page
ARTICLE TYPE: Original article TITLE: Phytochemical profiling and antioxidant potential of methanol extract of Vitis vinifera L seeds. Running Title: GC-MS and Antioxidant analysis of grape seed methanol extract. Authors: Sindhuja Lakshmi1, Kesavaram Padmavathy2*, Thirunavukarasu UmaArasu3, Dhandapani Prabu4. 1Research scholar, Department of Microbiology, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education & Research, Chennai, Tamil Nadu, India. 2Professor, Department of Microbiology, Research Laboratory for Oral and Systemic Health, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education & Research, Chennai, Tamil Nadu, India. 3Assistant Professor, Department of Microbiology, Swamy Vivekanandha Medical College Hospital and Research Institute, Elayampalayam, Namakkal Dt, Tamilnadu, India 4Assistant Professor and Head, Department of Microbiology, Dr. ALM, PGIBMS, University of Madras, Chennai, Tamil Nadu, India. *Corresponding author: *Kesavaram Padmavathy Professor, Department of Microbiology, Research Laboratory for Oral and Systemic Health, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education & Research, Chennai, Tamil Nadu, India. E-mail: padmabakianath@gmail.com; Phone: +91 9884164212.
Recommended Citation
Lakshmi, Sindhuja; Padmavathy, Kesavaram Dr; UmaArasu, Thirunavukarasu Dr; and Prabu, Dhandapani Dr
(2024)
"Phytochemical profiling and antioxidant potential of methanol extract of Vitis vinifera L seeds.,"
Chulalongkorn Medical Journal: Vol. 68:
Iss.
4, Article 8.
DOI: https://doi.org/10.56808/2673-060X.5459
Available at:
https://digital.car.chula.ac.th/clmjournal/vol68/iss4/8