Chulalongkorn Medical Journal


Background: Alpha lipoic acid (LA) is potent anti-oxidant and anti-inflammation natural compound. To enhance the efficacy and stability of LA, LA-loaded calcium citrate nanoparticles were developed.Objective: To investigate the anti-inflammatory effects of LA-loaded calcium citrate nanoparticles (LA-NPs) in HaCaT keratinocytes.Methods: HaCaT cells were exposed under two inflammation stimuli conditions, lipopolysaccharide (LPS) and Pb(NO3)2, and then were treated with or without LA and LA-NPs for 24 h. Cell viability and pattern of cell death were evaluated. In addition, the expression levels of inflammatory cytokines including IL-1β, IL-6, and TNF-α as well as inflammatory mediator COX-2 were determined by real-time polymerase chain reaction.Results: LA and LA-NPs decreased the percentage of cell death in both LPS and Pb(NO3)2-induced conditions.In LPS-induced cells, LA and LA-NPs attenuated the fold of gene expression levels in IL-1β, IL-6, TNF-α, and COX-2. Decreased expression in proinflammatory cytokines, including IL-1β and IL-6, was also observed in Pb(NO3)2-induced cells.Conclusion: Our study demonstrated the anti-inflammatory effects of LA and LA-NPs on LPS and Pb(NO3)2- induced human keratinocytes.



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