Abstract
Background : Formaldehyde exposure leads to increased risk of cancer. Recentstudies reveal that salt solution can preserve human and animal tissuesfor analysis with high quality equal to formaldehyde.Objective : To compare the effectiveness of formaldehyde and sodium chloride saltsolutions in various concentrations for preservation by quantitativemeasurement of tissue discoloration, volume and weight of human liverand brain slices.Methods : Sections of liver and brain were obtained from ten deceased subjects,and preserved in 10%, 20%, 26% sodium chloride salt solution (weight/weight) and 10% formaldehyde (volume/volume). The discoloration,volume and weight of each sample were quantitatively measured bycolorimeter on the 1st day (before the preservation process), 3rd, 7th, 14th,28th, and 56th day of the embalming.Results : All the brain samples which were preserved in 10% sodium chloride saltsolution, decomposed and only 4 samples in the 20% sodium chloridesalt solution sample group survived until the 56th day of preservation.There was no statistically significant difference on the 56th and the 1st dayof preservation between 26% sodium chloride salt solution andformaldehyde. The liver samples which were preserved in 10% and 20%sodium chloride salt solutions tended to decompose. There was astatistically significant difference between 26% sodium chloride saltsolution and formaldehyde in A (red-green) and L (lightness) color(P - value < 0.001). This means the liver slices preserved in 26% sodiumchloride salt solution showed less discoloration than those in formaldehyde.Conclusions : It is shown that 26% sodium chloride salt solution is an appropriatealternative choice for human liver and brain slice preservation, since it isa non-carcinogenic substance and shows less discoloration thanformaldehyde.
DOI
10.58837/CHULA.CMJ.61.1.3
First Page
17
Last Page
30
Recommended Citation
Jumlongkul, Arnon and Traithepchanapai, Pongpon
(2017)
"Comparison between formaldehyde and salt solutions for preservation of human liver and brain slices,"
Chulalongkorn Medical Journal: Vol. 61:
Iss.
1, Article 3.
DOI: https://doi.org/10.58837/CHULA.CMJ.61.1.3
Available at:
https://digital.car.chula.ac.th/clmjournal/vol61/iss1/3