Abstract
Background Paracetamol or acetaminophen (N-acetyl-p-aminophenol; APAP) is a widely used as an analgesic and antipyretic drug. This drug has long time been considered as a safe drug, when used in the therapeutic levels. However, the adverse effects concerning long-term APAP usage at the non-hepatotoxicity dosage regarding the human central nervous system, in particular microglia, remains unclear. Objective To investigate the effect of short-term (6, 12 and 24 hr.) and long-term APAP treatments (1, 2, 3 and 4 weeks) on the expression of the pro-inflammatory cytokine IL-1β in human microglia. Design Experimental research Setting Department of Biochemistry and Department of Pathology Faculty of Medicine, Chulalongkorn University Materials and Methods Human microglial (CHME-5) cells were cultured in the presence of APAP at the concentration determined by MTS assay for 6, 12 and 24 hr., representing short-term treatment. As for long-term APAP treatment, the cells were cultured with APAP for 1, 2, 3 and 4 weeks. Cell pellets were subsequently collected and the expression of the IL-1β in human microglia was determined by Western Blotting. Results The study shows that long-term APAP treatment induces morphological changes in CHME-5 cells. An increase in the number of amoeboid phagocytic cells with shorten processes is demonstrated in the long-term APAP- treated cells since the second week of treatment. Also, the level of IL-1β expression is up-regulated in a time-dependent manner while the short-term treatment shows no alteration, compared to that of control cultured cells. Conclusions The results demonstrated that long-term paracetamol treatment activates human microglial cell morphological changes and up-regulates pro-inflammatory cytokine IL-1β expression.
DOI
10.58837/CHULA.CMJ.59.3.2
First Page
253
Last Page
263
Recommended Citation
Wongprom, Benjawan; Maneesri-le, Supang; and Thongtan, Thananya
(2015)
"Long-term paracetamol treatment induces interleukin-1β expression in human microglial cells,"
Chulalongkorn Medical Journal: Vol. 59:
Iss.
3, Article 2.
DOI: https://doi.org/10.58837/CHULA.CMJ.59.3.2
Available at:
https://digital.car.chula.ac.th/clmjournal/vol59/iss3/2
