•  
  •  
 
Chulalongkorn Medical Journal

Abstract

Background : Tissue engineering requires scaffold for supporting tissue construction.The scaffold materials affect the scaffold properties and the achievementof tissue construction. Human skin, which is abundant of extracellularmatrix, is a promising source for fabricating tissue engineering scaffolds.Objective : To determine extracellular matrix components in human dermal solutions,and characterize fundamental physical and biological properties ofthe scaffolds made from the human dermal solutions.Methods : Cadaveric human skin was prepared to be 3 fractions of dermal solutions,denoted as DS-1, DS-2 and DS-3. These dermal solutions were determinedfor the content of collagen and sulfated glycosaminoglycans (sulfatedGAGs). Tissue engineering scaffolds were prepared form these dermalsolutions and their properties were characterized comparing with scaffoldsfrom type I collagen, commercially provided by Sigma-Aldrich corporation.The characterized physical properties included pore structure andmechanical strength and the characterized biological properties includeddegradation by collagenase, cell attachment and cell proliferation ofhuman bone marrow-derived stem cells (human BMSCs).Results : The components in each fraction of dermal solutions are obviously different.The dermal solutions contained collagen 92.23, 79.07 and 161.68 µg/mgdry weight and contained sulfated GAGs 3.09 ± 0.51, 1.36 ± 0.39 and6.91 ± 0.87 µg/mg dry weight of DS-1, DS-2 and DS-3, respectively.The scaffolds from DS-3 had the smallest average pore size, the highestelastic modulus and the longest degradation time with significant difference(p <0.05) while those of the scaffolds from DS-1 and DS-2 are insignificantlydifferent. The scaffolds from type I collagen (Sigma®) provided the mostfavorable cell attachment with significant difference (p <0.05) whereas nosignificant difference were found among the scaffolds from all types ofhuman dermal solutions. The lowest cell proliferation was found inthe scaffolds from DS-3 with significant difference (p <0.05). The highestcell proliferation was found in the scaffolds from DS-2, the second wasthe scaffolds from type I collagen (Sigma®), and the third was the scaffoldsfrom DS-1 but no significant difference was found.Conclusion : Human dermal solutions can be prepared from human dermis and usedas raw materials for scaffold fabrication. The contents of collagen andsulfated GAGs in each type of the human dermal solutions are different,as a result, the properties of the scaffolds fabricated from each type ofthe human dermal solutions are also different. The scaffolds fromthe human dermal solutions support the proliferation of human BMSCswithout any sign of cytotoxicity. Even though the scaffolds from type Icollagen (Sigma®)) provided better cell attachment than all types ofthe scaffolds from human dermal solutions, the scaffolds from DS-2 providebetter cell proliferation.

Publisher

Faculty of Medicine, Chulalongkorn University

First Page

177

Last Page

187

Share

COinS