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The Thai Journal of Pharmaceutical Sciences

Abstract

Background: Callicarpa macrophylla is used in traditional medicine to treat many disorders including analgesic, antipyretic and anti-inflammatory. The aim of this study is to investigate the ethanolic and ethylacetate extracts of C. macrophylla leaves against rheumatoid arthritis (RA). Materials and Methods: Acute toxicity was studied in vivo to determine the toxic doses of the ethanolic and ethyl acetate extracts. Anti-rheumatic activity was also evaluated in vivo using formaldehyde and Complete Freund’s Adjuvant models in Wistar rats. After that paw volume was evaluated by the digital Vernier caliper and blood sample was taken by retro-orbital plexus route for the estimation of hematological marker and plasma was used for the estimation of liver, kidney, and inflammatory markers. Further, the histopathology of the joint tissue was done by sacrificing the under experimented animals. Results: At 200 and 400 mg/kg, the ethanol and ethyl acetate extracts were the most active against RA. There was dose dependent reduction in paw volume in both arthritic models as compared to the arthritic control. The results of biological markers and hematological parameters were restored which were altered due to the progression of arthritis. All these results were further supported with histopathology of joint tissue where the extracts performed a reversal in inflammation and hyperplasia of synovium. The results of this study concluded that C. macrophylla ethanol extracts have better anti-arthritic activity in comparison with ethyl acetate extracts. Conclusion: The results of the present study support the traditional use of the leaves of C. macrophylla and may possibly serve as prospective material for further development of safe new pharmacoactive antirheumatic agents.

Publisher

Faculty of Pharmaceutical Sciences, Chulalongkorn University

First Page

688

Last Page

695

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