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The Thai Journal of Pharmaceutical Sciences

Abstract

Introduction and Objectives: Kidney is a front line organ exposed to the toxicity of gentamicin. The objective of this study was to determine the biopotential of Delphinium denudatum Wall., root a significant medicinal plant, against gentamicin. Materials and Methods: By performing high-performance liquid chromatography, an in vitro antioxidant study was conducted against diphenylpicrylhydrazide (DPPH). In vivo nephroprotective activity was evaluated by administering solo administration of gentamicin (GM; 40 mg/kg b.wt. single dose s.c.) for a period of 1st–13th days followed by hydroalcoholic extract of D. denudatum (HAEDD; 200, 400, and 800 mg/kg b.wt. intragastrically) to the rats for 14th–23rd days. Results: Nephrotoxicity confirmed through biochemical analysis, oxidative cascades, and histopathological alterations in kidney architecture. Cotreatment of DPPH with HAEDD demonstrated intense in vitro antioxidant activity, representing that D. denudatum exhibits cytoprotective activity (IC50 = 8.136 μg/mL). The protective effect of HAEDD (800 mg/kg b.wt.) compared with GM-nephrotoxicity is attributed to its antioxidant phytochemicals by inhibiting increase levels of urea, uric acid, and serum creatinine levels. The level of thiobarbituric acid reactive substances, glutathione, superoxide dismutase, and catalase levels in renal tissues was significantly (P < 0.05) improved, while treatment with HAEDD improved damaged renal architecture. Conclusion: Results demonstrated that D. denudatum therapy amplified enzyme performance more efficiently, probably due to the presence of antioxidant phytochemicals.

Publisher

Faculty of Pharmaceutical Sciences, Chulalongkorn University

First Page

524

Last Page

534

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