The Thai Journal of Pharmaceutical Sciences
Abstract
Background: The objective of the present investigation was to design and evaluate the antiurolithiatic activity of extract of Tribulus terrestris, Bryophyllum pinnatum, and Cyperus rotundus on ethylene glycol-induced urolithiasis in Wistar albino rats using Box Behnken Design. To induce urolithiasis, 0.75% w/v ethylene glycol was administered orally to the rats for 28 days. Cystone 750 mg/kg was selected as the reference standard as a preventive dose. On the 28th day, urine discharged in 24 h was collected and subjected for estimation of various biochemical parameters which were evaluated using urine, serum, and kidney homogenate. The kidneys were also sectioned and examined histopathologically to study the kidney architecture and calcium oxalate deposits. Results: The TT: BP: CR treatment significantly (P < 0.05) restored the parameters in urine, serum, and kidney homogenate to a near-normal level. An effective reduction in the stone promoter and an increase in stone inhibitor levels were observed. The optimum efficacy was observed in test group 13 with (TT: BP: CR= 50:100:35 mg/kg p.o) dosage regimen. The histopathological examinations revealed that calcium oxalate crystal deposits in the renal tubules and congestion and dilation of the parenchymal blood vessels were significantly reverted after the treatment with the herbal extract. Conclusions: The treatment with polyherbal formulation has the potential to prevent urolithiasis.
Publisher
Faculty of Pharmaceutical Sciences, Chulalongkorn University
First Page
432
Last Page
440
Recommended Citation
Gandhi, Tejal; Patel, Vaidehi; Dalwadi, Saloni; and Thakkar, Vaishali
(2022)
"Development and optimization of polyherbal formulation for antiurolithiatic activity using box behnken statistical design,"
The Thai Journal of Pharmaceutical Sciences: Vol. 46:
Iss.
4, Article 9.
Available at:
https://digital.car.chula.ac.th/tjps/vol46/iss4/9