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The Thai Journal of Pharmaceutical Sciences

Abstract

Background: Tithonia diversifolia has been widely developed as an anticancer therapy. Our previous study before showed that T. diversifolia was able to inhibit cell proliferation and collagen accumulation of keloid fibroblasts and decrease the index of rabbit scar hypertrophy. The acute dermal toxicity study of T. diversifolia gel formulation did not show toxic effect for 14 days and the LD50 was estimated more than 2%. However, toxicity data subchronic dermal toxicity study was not available for this T. diversifolia gel formulation. Objectives: Our study aimed to evaluate the subchronic dermal toxicity of T. diversifolia gel formulation in rats. Materials and Methods: T. diversifolia gel formulation with three dose variations of 2%, 10%, and 25% was administered topically. The lowest dose of 2% is the dose that has been used to decrease the index of rabbit scar hypertrophy. We observed the toxic effect that may appear every day for 90 days period. Results: There were no significant toxic effects observed at all doses on physical signs and symptoms, body weight, food consumption, hematological parameters, biochemical parameters, and macroscopic and microscopic examination organs. Conclusion: The analysis no observed adverse effect level showed that the administration long term of T. diversifolia gel formulation topically is safe to use within its dose recommendation.

Publisher

Faculty of Pharmaceutical Sciences, Chulalongkorn University

First Page

391

Last Page

398

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