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The Thai Journal of Veterinary Medicine

Abstract

Serum microRNAs (miRNAs) serve as diagnostic and prognostic biomarkers for various diseases. The serum concentration of miRNA-375 (miR-375), which is abundantly expressed in pancreatic islet cells, is increased in dogs with experimentally induced pancreatic injury and naturally occurring acute pancreatitis. However, this has not been reported in dogs with diabetes mellitus (DM). This study aimed to compare the expression of serum cfa-miR-375 between dogs with DM and healthy dogs and examine changes in serum cfa-miR-375 levels after insulin administration in dogs with DM. Twenty dogs with DM and 18 healthy dogs were included. The relative expression of serum cfa-miR-375 using reverse transcription and real-time PCR were evaluated. The primary endpoint was the comparison of serum cfa-miR-375 expression between dogs with DM and healthy dogs. The mean ± standard deviation (SD) fold change (FC) of serum cfa-miR-375 was significantly higher (P = 0.048) in dogs with DM (2.30 ± 2.018) than in healthy dogs (1.294 ± 0.560). The FC of serum miR-375 was significantly increased (P = 0.01) after treatment (4.017 ± 2.054) than before treatment (2.322 ± 2.608) in dogs with DM. The percentage change in cfa-miR-375 levels was positively correlated with the concentration of serum fructosamine post-treatment (r = 0.62, P = 0.01). Increased serum miR-375 levels may be associated with direct leakage from the damaged pancreas and pathological glucose regulation in canine DM.

DOI

10.56808/2985-1130.3951

First Page

1

Last Page

6

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