Molecular identification of Cryptosporidium spp. isolated from dairy calves in the Northern region of Algeria

Authors

Dadda Anes, Animal Production, Biotechnology and Health Laboratory, Department of Veterinary Sciences, Institute of Agronomic and Veterinary Sciences, Mohamed Cherif Mesaadia University
Abdellah Mohamed Cherif, Institut of Nature and Life Sciences, Tissemsilt UniversityFollow
Aoun Leila, Laboratory of Epidemiosurveillance, Health, Production, Reproduction, Experimentation and Cell Therapy of Domestic and Wild Animals. Chadli Benjdid University - ElTaref
Chatanun Eamudomkarn, Department of Parasitology, Faculty of Medicine, Khon Kaen University
Khelef Djamel, Higher National Veterinary School, PB 161 Rue Issad Abbes, OuedSmar, Algiers, Algeria, and Animal Health and Production Laboratory (AHPL), (NVS)
Keeseon S Eom, Department of Parasitology, Parasitology Research Center and International Parasite Resource Bank, Chungbuk National University, School of Medicine
Dongmin Lee, Department of Parasitology, Parasitology Research Center and International Parasite Resource Bank, Chungbuk National University, School of Medicine
Barakaeli Abdieli Ndosi, Department of Parasitology, Parasitology Research Center and International Parasite Resource Bank, Chungbuk National University, School of Medicine
Mohammed Mebarek Bia, Department of Parasitology, Parasitology Research Center and International Parasite Resource Bank, Chungbuk National University, School of Medicine

Abstract

Cryptosporidium spp. is the most frequent agent in Algeria that causes neonatal diarrhea in calves, with high symptomatic diarrhea in pre-weaned dairy calves. The study aims to measure the prevalence, distribution, and genotype of Cryptosporidium-infected calves in one of Algeria's high milk production areas (North Central and Northeast). From 35 dairy cattle farms were selected 172 (77 with diarrheic and 95 without diarrheic symptoms) calves aged less than 60 days. Twenty-three farms (65.71%) were detected with cryptosporidiosis using a modified Ziehl-Neelsen stain, whereby 68 calves (39.53%) were obtained positive. The Copro-ELISA test gave an individual prevalence of (37.66%, 22.11%) in calves with diarrhea and asymptomatic calves, respectively. Fifty positive cases confirmed with the previous tests reconfirmed by the target of S18 gene (SSU rRNA), in addition to the sequencing, were obtained from species C. parvum in all the analyzed samples. The subgenotype of C. parvum was obtained by amplifying the target gene of gp60, successfully confirmed by C. parvum type II (IIaA15G2R, IIaA16G2R1, and IIaA17G2R1). Our results confirmed the frequency of cryptosporidiosis in dairy calves from farms in Northern Algeria, with a pathogenic role in the calves, with potential zoonotic risk from food contamination.

DOI

10.56808/2985-1130.3865

First Page

1

Last Page

8

Recommended Citation

Anes, Dadda; Cherif, Abdellah Mohamed; Leila, Aoun; Eamudomkarn, Chatanun; Djamel, Khelef; S Eom, Keeseon; Lee, Dongmin; Ndosi, Barakaeli Abdieli; and Bia, Mohammed Mebarek (2025) "Molecular identification of Cryptosporidium spp. isolated from dairy calves in the Northern region of Algeria," The Thai Journal of Veterinary Medicine: Vol. 55: Iss. 4, Article 6.
DOI: https://doi.org/10.56808/2985-1130.3865
Available at: https://digital.car.chula.ac.th/tjvm/vol55/iss4/6