Extended-release buprenorphine with bupivacaine effectively provides postoperative analgesia in a mouse laparotomy model

Authors

Monika K. Huss, Department of Comparative Medicine, Stanford University School of Medicine, Stanford, CA, USAFollow
Amber Kerk, USDA-APHIS, Madison, WI, USAFollow
Renee Cotton, Department of Comparative Medicine, Stanford University School of Medicine, Stanford, CA, USAFollow
Helen Chum, In Vivo Strategies, San Francisco, CA, USAFollow
Michael Hsieh, Division of Urology, Children's National Hospital, The George Washington University, Washington DC, USAFollow
Katechan Jampachaisri, Department of Mathematics, Naresuan University, Phitsanulok, ThailandFollow
Patrick Sharp, Animal Resources, Purdue University, West Layfette, IN, USAFollow
Cholowat Pacharinsak, Department of Comparative Medicine, Stanford University School of Medicine, Stanford, CA, USAFollow

Abstract

Multimodal analgesia is widely encouraged for major survival surgery. This facilitates lower doses of individual drugs, thereby reducing side effects. Extended-release analgesics offer sustained analgesia coverage, prevent drug dosing nadirs, and reduce stress. Studies evaluating multimodal and extended-release analgesia combinations for major survival surgery in mice are limited. We compared the analgesic effect of bupivacaine and extended-release buprenorphine (Bupi-ER) to bupivacaine and buprenorphine HCl (Bupi-HCl). We hypothesized that preoperative Bupi-ER would provide more effective analgesia than Bupi-HCl. Mice were randomly assigned to 1 of 2 treatment groups: bupivacaine (2 mg/kg, local infiltration, once) with either 1) extended-release buprenorphine (0.6 mg/kg, SC once, n=6, Bupi-ER), or 2) buprenorphine HCl (0.1 mg/kg SC, BID, 2 days, n=5, Bupi-HCl). Animals underwent a laparotomy on day 0. Behavioral evaluations were performed twice a day by 2 blinded evaluators on day -1, 0, 1, and 2 post-operatively utilizing a modified Mouse Grimace Scale (mMGS) and behavioral scoring. On day 3, mice were sacrificed and weighed for experimental tissue collection. The mMGS and behavioral scores observed on D1 and D2 showed no significant difference compared to those on D-1 or D0. Our findings indicate that both Bupi-ER and BupiHCl provide effective multimodal analgesia in a mouse laparotomy model.

DOI

10.56808/2985-1130.3840

First Page

1

Last Page

8

Recommended Citation

Huss, Monika K.; Kerk, Amber; Cotton, Renee; Chum, Helen; Hsieh, Michael; Jampachaisri, Katechan; Sharp, Patrick; and Pacharinsak, Cholowat (2025) "Extended-release buprenorphine with bupivacaine effectively provides postoperative analgesia in a mouse laparotomy model," The Thai Journal of Veterinary Medicine: Vol. 55: Iss. 3, Article 4.
DOI: https://doi.org/10.56808/2985-1130.3840
Available at: https://digital.car.chula.ac.th/tjvm/vol55/iss3/4