Biocompatibility and anti-inflammatory effects of Thai cannabidiol-containing topical anesthetic gel prototype in a dermal irritation test

Authors

Pasutha Thunyakitpisal, Institute of Dentistry, Suranaree UniversityFollow
Supattarayan Thongjit, Institute of Dentistry, Suranaree UniversityFollow
Apitchaya Pruksametanan, Institute of Dentistry, Suranaree UniversityFollow
Wijit Banlunara Assoc. Prof., Center of Excellence in Materials and Bio-Interfaces, Chulalongkorn UniversityFollow

Abstract

The objective of this study is to investigate and compare the effect of cannabidiol (CBD)-containing and non-CBD topical anesthetic gel prototypes on skin irritation in rabbits at 24, 48, and 72 h post-application. Topical anesthetic gel prototypes containing 0.1% CBD and without CBD were developed. Six rabbits (Oryctolagus cuniculus) were used for skin irritation testing according to ISO 10993-23: 2021. The animals were randomly and equally divided into two groups (CBD and non-CBD gel groups). The gels were applied to the animals’ skin at the dorsal area of the flank with good contact for 4 h. Distilled water was used as the control. After exposure, skin reactions (erythema and oedema scores), the primary irritation score, and the primary irritation index were evaluated immediately and at 24, 48, and 72 h postapplication. Data were analyzed using the SPSS program with a paired t-test (P < 0.05). Both the CBD and non-CBD groups exhibited mild skin irritation. However, the CBD group showed a lower primary irritation index, particularly at the 72-h time point. In conclusion, the CBD-containing topical anesthetic prototype has mild skin irritation. CBD may help alleviate the irritating effects of topical local anesthetics.

DOI

10.56808/2985-1130.3903

First Page

1

Last Page

6

Recommended Citation

Thunyakitpisal, Pasutha; Thongjit, Supattarayan; Pruksametanan, Apitchaya; and Banlunara, Wijit Assoc. Prof. (2025) "Biocompatibility and anti-inflammatory effects of Thai cannabidiol-containing topical anesthetic gel prototype in a dermal irritation test," The Thai Journal of Veterinary Medicine: Vol. 55: Iss. 3, Article 15.
DOI: https://doi.org/10.56808/2985-1130.3903
Available at: https://digital.car.chula.ac.th/tjvm/vol55/iss3/15