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The Thai Journal of Veterinary Medicine

Abstract

Subclinical babesiosis observed in captive Eld’s deer, which act as reservoirs for ticks, poses a risk to healthy herds. However, the knowledge of biomarkers for subclinically infected Eld’s deer and host-parasite interactions is limited. This study aimed to investigate the serum proteomic profiles and protein interactions of Babesia bovis and subclinically infected Eld’s deer, PCR-positive for B. bovis, using automated serum protein electrophoresis and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The findings revealed albumin and four globulin fractions: alpha-1, alpha-2, beta, and gamma globulin. Albumin protein (ALB) was consistently detected across all fractions, highlighting its role in maintaining osmotic balance and as a carrier protein. The study also identified scaffold protein involved in DNA repair (SPIDR), a genome stability-maintaining protein, in all globulin fractions. Eld’s deer proteins, such as phospholipid transfer protein C2CD2L (C2CD2L), serpin A3 (SERPINA3), and various immune-related proteins were expressed, indicating a host response to B. bovis infection. Protein interaction network analysis revealed that the alpha-1 and beta globulin fractions were enriched in pathways associated with the acute phase response and immune defense. Additionally, interactions within the gamma globulin fraction were enriched in telomerase RNA binding, emphasizing cell proliferation. Additionally, Eld’s deer C2CD2L interacted with B. bovis heat shock protein 70 (HSP70), indicating that B. bovis could potentially stimulate the immune response of the infected host. In summary, this study provides insights into the molecular mechanisms of host-pathogen interactions, highlighting the complexity of immune responses during B. bovis infection.

DOI

10.56808/2985-1130.3720

First Page

155

Last Page

173

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