Protective Immunity of the Pore-Forming Domains of Actinobacillus pleuropneumoniae Apx Toxins in a Mouse Model

Authors

Kamonpun Chuekwon, Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and TechnologyFollow
Li-Ting Cheng, Graduate Institute of Animal Vaccine Technology, College of Veterinary Medicine, National Pingtung University of Science and TechnologyFollow
Chun-Yen Chu, International Program in Animal Vaccine Technology, International College, National Pingtung University of Science and TechnologyFollow

Abstract

The hemolytic/leukolytic toxins ApxI, II, and III play a central role in the pathogenicity of Actinobacillus pleuropneumoniae and may serve as effective vaccine antigens. However, since these toxins are large in size, we aimed to determine whether only the pore-forming domain of the toxins may be sufficient for protective immunity. Mice were vaccinated with the pore-forming domains for immune response analysis and challenge test. Significant antibody response was observed for the ApxI and II vaccine groups. For cellular immune response, CD4⁺ and CD8⁺ T cell expansion was observed for ApxI. Pro-inflammatory cytokine (IL-1b, IL-6) and T_H2-type cytokine (IL-4, IL-10) gene expression was detected for the ApxI and II groups. Finally, in a challenge test with a A. pleuropneumoniae serotype 1 strain, the pore-forming domains of ApxI, II, III and three of pore-forming domains conferred 100%, 40%, 0% and 100% protection, respectively. The pore-forming domain of the Apx toxins shows promise as vaccine antigen against A. pleuropneumoniae.

DOI

10.56808/2985-1130.1057

First Page

73

Last Page

81

Recommended Citation

Chuekwon, Kamonpun; Cheng, Li-Ting; and Chu, Chun-Yen (2024) "Protective Immunity of the Pore-Forming Domains of Actinobacillus pleuropneumoniae Apx Toxins in a Mouse Model," The Thai Journal of Veterinary Medicine: Vol. 53: Iss. 1, Article 7.
DOI: https://doi.org/10.56808/2985-1130.1057
Available at: https://digital.car.chula.ac.th/tjvm/vol53/iss1/7