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The Thai Journal of Veterinary Medicine

Abstract

In vitro spermatogenesis offers a therapeutic modality for the treatment of male infertility associated with pre-or post-meiotic barriers to the spermatogenesis process. The present study reports the production of tail-bearing sperm-like cells from in vitro spermatogenesis of farming goat testis in an air-liquid inter-phase culture. The testicular fragments (1-2 mm3) were placed on agarose scaffolds at air-liquid interphase and cultured in vitro for 60 days. Proliferating germ cell colonies were visible after 45-55 days of culture. Differentiation of cells to spermatogonia and spermatocytes was observed from the 40th day onwards. Tail bearing sperm-like elongated cells, with apical acrosome, were detected from the 45th day onwards. The differentiating germ-line cells were confirmed by stage-specific expression of positive markers (VASA, DAZL, SYCP1, SYCP3, C-Kit, POU5F1 and SOX-2). Histological sections of the cultured testicular tissue further confirmed the presence of tail-bearing sperm-like cells in the lumen of seminiferous tubules which was absent in non-cultured tissue. In conclusion, our study suggests that the air-liquid interphase organ culture might be useful in vitro generation of sperm in farming goats. The study could have a significant impact on the livestock management of the farmers and could improve their dependency on orthodox and natural breeding management.

Publisher

Faculty of Veterinary Science, Chulalongkorn University

First Page

473

Last Page

484

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