The Thai Journal of Veterinary Medicine


ALV-J is an important pathogen in the poultry industry in China. In this study, we developed an immunochromatographic test (ICT) assay for the detection of antibody against of ALV-J to evaluate ALV-J infection. Gp85 protein of ALV-J was employed as a capture antigen. Antigen-antibody reaction was detected by either colloidal-gold conjugated gp85 protein or monoclonal antibody of ALV-J. The high consistency of results in ICT and ELISA indicated that the ICT is reliable. This suggests that the gp85-based ICT can be used as a rapid on-site diagnostic method of ALV-J.

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