Immunochromatographic test (ICT) for the detection of antibody against ALV-J

Authors

Guihua Wang
Yanming Wang
Ziqiang Cheng

Abstract

ALV-J is an important pathogen in the poultry industry in China. In this study, we developed an immunochromatographic test (ICT) assay for the detection of antibody against of ALV-J to evaluate ALV-J infection. Gp85 protein of ALV-J was employed as a capture antigen. Antigen-antibody reaction was detected by either colloidal-gold conjugated gp85 protein or monoclonal antibody of ALV-J. The high consistency of results in ICT and ELISA indicated that the ICT is reliable. This suggests that the gp85-based ICT can be used as a rapid on-site diagnostic method of ALV-J.

DOI

10.56808/2985-1130.3227

First Page

373

Last Page

376

Recommended Citation

Wang, Guihua; Wang, Yanming; and Cheng, Ziqiang (2022) "Immunochromatographic test (ICT) for the detection of antibody against ALV-J," The Thai Journal of Veterinary Medicine: Vol. 52: Iss. 2, Article 17.
DOI: https://doi.org/10.56808/2985-1130.3227
Available at: https://digital.car.chula.ac.th/tjvm/vol52/iss2/17