Possibility of trypsin treated dog erythrocytes as universal blood: evaluation by FTIR microspectroscopy

Authors

Pornphimon Metheenukul
Kanjana Thumanu
Thanit Metheenukul

Abstract

A common canine erythrocyte antigen is DEA 1.1 but it is the most immunogenic. In this study, Fourier transformed infrared (FTIR) microspectroscopy was used to examine the alterations of membrane biomolecules in trypsin-treated DEA 1.1+ canine erythrocytes. Production of canine DEA 1.1- erythrocytes was achieved by treating DEA 1.1+ red cells (5% cell suspension) with 1 mg/ml trypsin for 120 mins, without affecting the intracellular 2,3-diphosphoglycerate level. FTIR microspectroscopy was employed to study the effects of trypsin-treatment on canine trypsinized red cell membrane components. Production of canine DEA 1.1- erythrocytes was achieved without affecting the intracellular 2,3-diphosphoglycerate level. The FTIR microspectroscopy data suggested that the trypsin treated dog erythrocytes were possible for use as universal blood.

DOI

10.56808/2985-1130.3124

First Page

321

Last Page

329

Recommended Citation

Metheenukul, Pornphimon; Thumanu, Kanjana; and Metheenukul, Thanit (2021) "Possibility of trypsin treated dog erythrocytes as universal blood: evaluation by FTIR microspectroscopy," The Thai Journal of Veterinary Medicine: Vol. 51: Iss. 2, Article 15.
DOI: https://doi.org/10.56808/2985-1130.3124
Available at: https://digital.car.chula.ac.th/tjvm/vol51/iss2/15