The Oroxylum indicumplant is aherbal plant commonly eaten by the locals in Malaysia while the application of herbal remedies from the plant has been inherited and passed down through generations. However, there is a lack of toxicity profiling of the plant, hence this research aimed toinvestigate acute oral toxicity ofethanol extract of O. indicumin C57BL/6 male mice at different concentrations, to determine the LD50of the plant extract. A total of twenty-five mice were randomly assigned into five experimental groups comprising the control (normal saline), vehicle (5% DMSO), low dose (1000 mg/kg bw), medium dose (2000 mg/kg bw) and high dose (5000 mg/kg bw). The extracts were administered in a single oral dose on day 1 and the mice were observed daily for mortality, physiological andbehavioural changes throughout the 14 day study period. At the end of the study, vital organs and blood samples were collected to determine the effects of the extract on the relative organ weight, tissue changes and blood profile alterations.No mortalitynor behavioural changes were recorded for 2 weeks. Results of the body weight, relative organ weight, haematological and serum biochemistry assessments showed no significant (p>0.05) changes. Nevertheless, there were significant differences in the mean corpuscular volume (MCV), urea and alanine transaminase (ALT) values but the levels were still within the normal range. Histopathological analysis of the liver and kidney tissues also revealed no striking lesions. In summary, this study indicates that O. indicumleaf ethanolic extract up to 5000 mg/kg bw did not cause any toxicological effects in the mice model and is safe to be used for therapeutic purposes.
Farhan Hanif Reduan, Mohd; Hamid, Fathin Faahimaah Abdul; Nordin, Muhammad Luqman; Shaari, Rumaizi; Hamdan, Ruhil Hayati; Teik Chung, Eric Lim; Peng, Tan Li; Kamaruzaman, Intan Noor Aina; and Noralidin, Noramalina
"Acute oral toxicity study of ethanol extract of Oroxylum indicum leaf in mice,"
The Thai Journal of Veterinary Medicine: Vol. 50:
4, Article 17.
Available at: https://digital.car.chula.ac.th/tjvm/vol50/iss4/17