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The Thai Journal of Veterinary Medicine

Abstract

Mycoplasma bovis(M. bovis) is one of the most significant pathogens in cattle. The aim of this study was to develop a rapid real-time LAMP assay, specific for M. boviswith designed primers targeting the OPPD/F gene. The assay was tested for 1 h at 63°C. The results could be visualized by the addition of calceindye. Real-time LAMP was specific exclusively for M. boviswithout cross-reactivity with other bacteria. The sensitivity of the real-time LAMP was determined to be 2.29×10-3ng/μL of DNA and was 100 times higher than conventional PCR. Lamp assay was conducted on 65 clinical samples to test its usefulness and reliability and compared to the PCR assay results. A concordance of 100% was observed between the two assays. This real-time LAMP assay can be applied by diagnostic laboratories and for field detection of M. bovisinfection

Publisher

Faculty of Veterinary Science, Chulalongkorn University

First Page

363

Last Page

369

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