The Thai Journal of Veterinary Medicine
Article Title
Rapid detection of Mycoplasma bovisby real-time Loop-mediated isothermal amplification
Abstract
Mycoplasma bovis(M. bovis) is one of the most significant pathogens in cattle. The aim of this study was to develop a rapid real-time LAMP assay, specific for M. boviswith designed primers targeting the OPPD/F gene. The assay was tested for 1 h at 63°C. The results could be visualized by the addition of calceindye. Real-time LAMP was specific exclusively for M. boviswithout cross-reactivity with other bacteria. The sensitivity of the real-time LAMP was determined to be 2.29×10-3ng/μL of DNA and was 100 times higher than conventional PCR. Lamp assay was conducted on 65 clinical samples to test its usefulness and reliability and compared to the PCR assay results. A concordance of 100% was observed between the two assays. This real-time LAMP assay can be applied by diagnostic laboratories and for field detection of M. bovisinfection
First Page
363
Last Page
369
Recommended Citation
Pan, Yan; Wei, Ying Ming; AE, Garmendia; Peng, Hao; Feng, Shi Wen; Tao, Li; and Li, Jun
(2020)
"Rapid detection of Mycoplasma bovisby real-time Loop-mediated isothermal amplification,"
The Thai Journal of Veterinary Medicine: Vol. 50:
Iss.
3, Article 9.
DOI: https://doi.org/10.56808/2985-1130.3038
Available at:
https://digital.car.chula.ac.th/tjvm/vol50/iss3/9