The Thai Journal of Veterinary Medicine
Article Title
Rapid detection of Mycoplasma bovisby real-time Loop-mediated isothermal amplification
Abstract
Mycoplasma bovis(M. bovis) is one of the most significant pathogens in cattle. The aim of this study was to develop a rapid real-time LAMP assay, specific for M. boviswith designed primers targeting the OPPD/F gene. The assay was tested for 1 h at 63°C. The results could be visualized by the addition of calceindye. Real-time LAMP was specific exclusively for M. boviswithout cross-reactivity with other bacteria. The sensitivity of the real-time LAMP was determined to be 2.29×10-3ng/μL of DNA and was 100 times higher than conventional PCR. Lamp assay was conducted on 65 clinical samples to test its usefulness and reliability and compared to the PCR assay results. A concordance of 100% was observed between the two assays. This real-time LAMP assay can be applied by diagnostic laboratories and for field detection of M. bovisinfection
Publisher
Faculty of Veterinary Science, Chulalongkorn University
First Page
363
Last Page
369
Recommended Citation
Pan, Yan; Wei, Ying Ming; AE, Garmendia; Peng, Hao; Feng, Shi Wen; Tao, Li; and Li, Jun
(2020)
"Rapid detection of Mycoplasma bovisby real-time Loop-mediated isothermal amplification,"
The Thai Journal of Veterinary Medicine: Vol. 50:
Iss.
3, Article 9.
Available at:
https://digital.car.chula.ac.th/tjvm/vol50/iss3/9