Rapid detection of Mycoplasma bovisby real-time Loop-mediated isothermal amplification

Authors

Yan Pan
Ying Ming Wei
Garmendia AE
Hao Peng
Shi Wen Feng
Li Tao
Jun Li

Abstract

Mycoplasma bovis(M. bovis) is one of the most significant pathogens in cattle. The aim of this study was to develop a rapid real-time LAMP assay, specific for M. boviswith designed primers targeting the OPPD/F gene. The assay was tested for 1 h at 63°C. The results could be visualized by the addition of calceindye. Real-time LAMP was specific exclusively for M. boviswithout cross-reactivity with other bacteria. The sensitivity of the real-time LAMP was determined to be 2.29×10-3ng/μL of DNA and was 100 times higher than conventional PCR. Lamp assay was conducted on 65 clinical samples to test its usefulness and reliability and compared to the PCR assay results. A concordance of 100% was observed between the two assays. This real-time LAMP assay can be applied by diagnostic laboratories and for field detection of M. bovisinfection

DOI

10.56808/2985-1130.3038

First Page

363

Last Page

369

Recommended Citation

Pan, Yan; Wei, Ying Ming; AE, Garmendia; Peng, Hao; Feng, Shi Wen; Tao, Li; and Li, Jun (2020) "Rapid detection of Mycoplasma bovisby real-time Loop-mediated isothermal amplification," The Thai Journal of Veterinary Medicine: Vol. 50: Iss. 3, Article 9.
DOI: https://doi.org/10.56808/2985-1130.3038
Available at: https://digital.car.chula.ac.th/tjvm/vol50/iss3/9