The Thai Journal of Veterinary Medicine


This article presents modern methods for obtaining and isolatingpure culture from bee colonies (from honeycombswith infected broodsand contaminated by faeces of bees), and the identification and indication of pathogenic bacteria of Klebsiellaaerogenes species in honey beesduring the winter, spring,summer andthe autumn. The purposewas to isolate and identifythe pure culture of enterobacteria from the pathological material of diseased bee colonies. The study of their basic biological properties for the possible use of the obtained strains, which should be used as indicator bacterial cultures in the study of the biological properties of drugs for the treatment and prevention of enterobacteriosis of beeswas undertaken.The etiological factor for the origin of the collapse of bee colonies belongs to the Family Enterobacteriaceae, the genus being Klebsiella –Klebsiella aerogenes (previously named Enterobacter aerogenes), which was established by itscultural properties(Endo –light pink, small, flat, mucous, matte colonies; Levin –light pink, flat, mucous, matte colonies);by morphological properties (short, sticks, small, placed together and singly, without capsules, mobile);by tinctorial (gram negative) properties and by results of biochemical typing of microorganisms(Kligler medium: glucose –negative, acid-gas H2S negative; Simons medium –positive, acetate Na –positive, malonate Na –negative; Phenylalanine –negative: Indole –negative; Urease –negative), which wereisolated from the intestines of bees and their faeces from the frames of bee colonies

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