Molecular characterization of equine herpesvirus-1 and asinine herpesvirus -5 isolated from aborted fetuses of arabian horses

Authors

Rabab Taha Hassanien
Ehab Mostafa El-Nahas
Nahed Ahmed Mahmoud
Gabr Fikry El-Bagoury

Abstract

Infections with equine herpesviruses(EHVs) are widespread in equine populations worldwide. Whilst EHV-1 produces well-documented reproductive losses in equids, the role of asinineherpesviruses-5 (AHV-5) to induce abortion is still enigmatic. In the present study, EHV-1 and AHV-5 were detected in aborted fetuses of Arabian horses in Egypt by consensus nested PCR of DNA polymerase gene, with a trial for viral isolation on Rabbit Kidney 13 cell line (RK-13) and identification by indirect immunofluorescence. The partial analysis of DNA polymerase gene sequence revealed that the Egyptian strains EHV-1 and AHV-5 were related to Australian origin EHV-1 838/89 (Accession. no, KF434370) and Austrian strain AHV-5 isolate 1323 (Accession. no, KC825357) by 98.3% and 98.6 % respectively. In conclusion, this was the first detection and isolation of AHV-5 in Egypt, and to our knowledge, this represents the first report of AHV-1 detection from an aborted fetus worldwide.

DOI

10.56808/2985-1130.3042

First Page

397

Last Page

403

Recommended Citation

Hassanien, Rabab Taha; El-Nahas, Ehab Mostafa; Mahmoud, Nahed Ahmed; and El-Bagoury, Gabr Fikry (2020) "Molecular characterization of equine herpesvirus-1 and asinine herpesvirus -5 isolated from aborted fetuses of arabian horses," The Thai Journal of Veterinary Medicine: Vol. 50: Iss. 3, Article 13.
DOI: https://doi.org/10.56808/2985-1130.3042
Available at: https://digital.car.chula.ac.th/tjvm/vol50/iss3/13