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The Thai Journal of Veterinary Medicine

Abstract

Anaemia, emaciation, increased mortality and decreased egg production were observed in 21 birds in a Chinese local breed flock infected naturally at 240 days old. Macroscopically, the spleen and kidneys were enlarged. There were diffuse, multifocal white foci on the surface of the liver, lungs and ovaries. Histopathological examination demonstrated typical lymphosarcoma cells and erythroblasts in the lungs, spleen, liver, kidneys and ovaries. The results of polymerase chain reaction (PCR) showed that avian leukosis virus subgroup J(ALV-J) was positive in the liver, spleen and ovaries. Immunofluorescence assay results further confirmed the presence of ALV-J antigen in the liver, kidneys and ovaries. Sequence analysis of the specific nucleotides fragments demonstrated that the viruses were closest to the United Kingdom (UK) prototype strain virus HPRS-103 (97.8% to 98.2%). This is the first report of erythroblasts and lymphoblastscaused by ALV-J in Chinese local breed chickens.

First Page

277

Last Page

281

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