The Thai Journal of Veterinary Medicine


Animal preservation for anatomy teaching has been required to replace formaldehyde and phenolwhich represent a health hazard to humans. A comparative study of embalming solutions with partial replacement of formaldehyde and phenol with sodium chloride and ethanol was evaluated in mouse cadavers over an 18-month period. Twelve adult mouse carcasses were divided into six groups of two mice each. After openingthe mouse’s skin,the internal organswere removed and preserved with the remaining cadavers by freezing (Group I, control group) or in the respective embalming solution (Groups II to VI) and examined after 3, 6, 12 and 18 months. For the embalming solution, the formaldehyde concentration wasreduced from 18% (Group II) to 0.5% (Groups III and IV), 0.1% (Group V), and 0.05% (Group VI), whereas the phenol and glycerol concentration was reduced from 2.5% and 5%(Group II) to 0.8% and 2%, (Groups III-VI), respectively. In replacement, 15% tablesalt/sea salt and 16% ethanol were included in Groups III and IV. The carcasses and internal organs were blind evaluated by 10 students. The evaluationswere compared and analyzed between Groups and against Group 1using ANOVA. Results showthat embalmed mice in Groups III and IV retained a good color and flexibility, without decomposition or fungal growththroughout study. In contrast, mice in Groups V and VI were unsuitable at 3 months. Weconcluded that asuitableembalming fluid for small animal preservation was0.5% formaldehyde, 0.8% phenol, 2% glycerol, 16% ethanol and 15%sodium chloride.



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