The Thai Journal of Veterinary Medicine


Salmonellaspp. can be widely spread by air or contaminated food. There are sixcommon pathogenic Escherichia coli whichaccount for 4–8% of all E. coli pathogens. Amongstthem, E. coliO157 is the most commonlyknown. In this study, we investigated the prevalence of diarrhoeapathogens, referred to as Salmonellaspp.andEscherichia coli(E. coli), in pet dogs in western Taiwan using the conventionalpolymerase chain reaction (PCR). The related analysis between the prevalence rates and the epidemiological data of pet dogs was correlated with age, season, area and breed. Faecalsamples were collected from240dogs which had symptoms of diarrhoeaand had been examined byveterinaryhospitals from January 2015 to January 2017. The PCR sensitivityof the total deoxyribonucleic acids (DNAs) extracted from 0.1g faecal samples ranging from 10 fgto 100 ng was examined. The prevalence of Salmonella spp. andE. coliinfections was19.2% (46/240) and 3.3% (8/240),respectively. Resultsshowed that Salmonellaspp.was at its most prevalent during spring,whilstE. coliwas most prevalent in summer, and the highest proportions might occur in puppiesin suburbandogs. Since both diseases are zoonotic, the more humans areexposed to it, the higher its occurrence will be. This study has provided clinical veterinarians with the advanced ability to diagnose both diseases and with crucial information for the prevalence of Salmonellaspp. andE. colidiseases in dogswith diarrhoeain Taiwan.



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